The interaction of Taurine and HIIT on PI3K/AKT and Txnip in mice C57BL/6 fed HFD

Background: Non-alcoholic fatty liver disease (NAFLD) has become one of the most prevalent diseases in recent decades, expanding alongside obesity in society. It typically begins with insulin resistance and progresses through the activation of inflammatory pathways.Purpose: The aim of this study was to explore the effects of taurine supplementation and high-intensity interval training (HIIT) on the TXNIP/NLRP3/CASPASE1 and PI3K/AKT/PTEN pathways in male miceC57BL/6 fed a high fat diet. Methods: Fifty-four male C57BL/6J mice were randomly divided into two groups: a control diet group (n=8) and a high-fat diet (HFD) group to induce NAFLD (n=48). After 12 weeks, HFD group further divided into six subgroups: HFD control (n=8), HFD+ HIIT (n=8), HFD+ 2.5%taurine supplementation (n=8), HFD+HIIT+ 2.5% taurine supplementation (n=8), HFD+ 5%taurine supplementation (n=8), and HFD+ HIIT+ 5%taurine supplementation (n=8). The exercise protocol consisted of 10 weeks of HIIT, five days per week, and taurine supplementation was administered in water solubility throughout the entire period.Results: There were significant differences between the control and HFD groups in terms of weight and liver enzymes levels. Additionally, a significant difference was observed in the HOMA2 index between HFD control and the other intervention groups. The HFD affected the PI3K/AKT/PTEN and TXNIP/NLRP3/CASPASE1 pathways. Taurine supplementation and HIIT had a positive impact on the phosphorylation of PI3K and AKT and expression of PTEN and also TXNIP and caspase1 reversing the effects of the HFD. results indicated that a lower dose of taurine had a stronger effect, particularly on the TXNIP pathway.Conclusion: In summary, combining HIIT with a lower dose of Taurine supplementation may be more effective than either intervention alone in mitigating the progression of NAFLD in male C57BL/6 mice.