Identification of LncRNAs Associated Proteins Using Mass Spectrometry-Based Proteomics Landscape for Unlocking the Secrets of Cancer

Background/Objectives: To develop a novel method for isolating proteins associated with Long Non-coding RNAs (LncRNAs) using high-throughput Liquid Chromatography Mass Spectrometry (LC-MS) and proteomics approaches for identifying cancer-associated proteins involved in post-translational modifications (PTMs) such as glycosylation, phosphorylation, citrullination, methylation, and acetylation. Additionally, the focus is on identifying and quantifying differentially modulated or regulated proteins associated with LncRNAs to discover novel molecular signatures or biomarkers. Methods: Identification, quantification, and characterization of LncRNAs associated pro-teins by using Label free Quantification (LFQ) of proteins by Nono LC-Mass Spectrometry (LC-MS)., use Employ bioinformatics, biostatistics analysis for find out the differentially expressed, exclusive protein or modulated., find outInvestigate the mechanisms and pathways involving these unique or specific proteins analysis of unique/specific proteins and Validation of these unique/specific proteins by Immunohistochemistry (IHC) and western blotting/ enzyme-linked immunosorbent assay (ELISA) for to achieve better out-come. Conclusions: Identified and validated novel proteins associated with LncRNAs could be early detective molecular biomarkers of cancer and it could be useful in clinical practice for diagnosis, prognosis of liver, colorectal and other several types of cancer types.