Kidney and Lung ACE2 Expression after an ACE Inhibitor or an Ang II Receptor Blocker: Implications for COVID-19
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SciScore for 10.1101/2020.05.20.106658: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement IACUC: Animal Models: All studies were conducted with the review and approval of the Institutional Animal Care and Use Committee of Northwestern University. Randomization Two other experimental groups of 12-14 weeks old C57BLKS/J mice were randomly assigned to drink either tap water (vehicle, n=6) or tap water with an angiotensin II receptor antagonist, telmisartan (Boehringer Ingelheim), at a dose of 2 mg*kg−1*day−1 (n=6) for 14 days. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Table 2: Resources
Antibodies Sentences Resources ACE2 protein was detected using our affinity purified rabbit anti-ACE2 antibody (obtained as … SciScore for 10.1101/2020.05.20.106658: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement IACUC: Animal Models: All studies were conducted with the review and approval of the Institutional Animal Care and Use Committee of Northwestern University. Randomization Two other experimental groups of 12-14 weeks old C57BLKS/J mice were randomly assigned to drink either tap water (vehicle, n=6) or tap water with an angiotensin II receptor antagonist, telmisartan (Boehringer Ingelheim), at a dose of 2 mg*kg−1*day−1 (n=6) for 14 days. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Table 2: Resources
Antibodies Sentences Resources ACE2 protein was detected using our affinity purified rabbit anti-ACE2 antibody (obtained as described in. anti-ACE2suggested: NoneExperimental Models: Organisms/Strains Sentences Resources ACE.4 mice have the somatic ACE promoter replaced by the kidney androgen-regulated protein promoter which in these mice is essentially non-functional(26); in the absence of exogenous androgens the levels of kidney ACE are less than 1% of normal and no ACE is detected in other organs; this model is overtly hypotensive (26). ACE.4suggested: NoneIn addition, ACE8/8 mice have significant levels of ACE activity in the lung and in the plasma and blood (27). ACE8/8suggested: NoneTo examine the effect of pharmacological ACE inhibition on ACE2, two groups of 12-14 weeks old C57BLKS/J mice were assigned to drink either tap water (n=8) or tap water with an ACE inhibitor, captopril, (n=8) at a dose of 120 mg*kg−1*day−1 for 14 days. C57BLKS/Jsuggested: NoneSoftware and Algorithms Sentences Resources Primers and probes for ACE were designed using Primer Express software (Applied Biosystems). Primer Expresssuggested: (Primer Express, RRID:SCR_014326)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We found bar graphs of continuous data. We recommend replacing bar graphs with more informative graphics, as many different datasets can lead to the same bar graph. The actual data may suggest different conclusions from the summary statistics. For more information, please see Weissgerber et al (2015).
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- No funding statement was detected.
- No protocol registration statement was detected.
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