Infection and transmission of SARS‐CoV‐2 depend on heparan sulfate proteoglycans

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1. Version published to 10.15252/embj.2020106765

   Sep 23, 2021
2. 

   ScreenIT

   Mar 1, 2021

   SciScore for 10.1101/2020.08.18.255810: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Institutional Review Board Statement	IRB: Alveolar macrophages were prepared from broncheo-alveolar lavage (BAL) fluid that was obtained as spare material from the ongoing DIVA study (Netherlands Trial Register: NL6318; AMC Medical Ethical Committee approval number: 2014_294). Consent: All subjects in the DIVA study have signed an informed consent form.
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Sex as a biological variable	The DIVA study includes healthy male volunteers aged 18-35.
   Cell Line Authentication	not detected.

   Table 2: Resources

   Antibodies
   Sentences	Resources
   Reagents and antibodies: The following antibodies were used (all anti-human): ACE-2 (R&D),	anti-human): ACE-2 su…

   SciScore for 10.1101/2020.08.18.255810: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Institutional Review Board Statement	IRB: Alveolar macrophages were prepared from broncheo-alveolar lavage (BAL) fluid that was obtained as spare material from the ongoing DIVA study (Netherlands Trial Register: NL6318; AMC Medical Ethical Committee approval number: 2014_294). Consent: All subjects in the DIVA study have signed an informed consent form.
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Sex as a biological variable	The DIVA study includes healthy male volunteers aged 18-35.
   Cell Line Authentication	not detected.

   Table 2: Resources

   Antibodies
   Sentences	Resources
   Reagents and antibodies: The following antibodies were used (all anti-human): ACE-2 (R&D),	anti-human): ACE-2 suggested: None
   Negative control included isotype-matched HIV-1 antibody VRC01 (59)	HIV-1 suggested: (bNAber Cat# bNAberID_1, RRID:AB_2491019)
   Purity of LCs was routinely verified by flow cytometry using antibodies directed against CD207 (langerin) and CD1a.	CD207 suggested: None CD1a suggested: None
   Experimental Models: Cell Lines
   Sentences	Resources
   Cell lines: The human B cell line Namalwa (ATCC, CRL-1432) and Namalwa cells stably expressing human Syndecan 1 and Syndecan 4 (57) were a gift from Dr. Guido David and Dr. Philippe A Gallay.	Namalwa suggested: None
   The human epithelial Caco2 cells (ATCC, HTB-37™) were maintained in Dulbecco modified Eagle medium (Gibco Life Technologies, Gaithersburg, Md.) containing 10% fetal calf serum (FCS), L-glutamine and penicillin/streptomycin (10 μg/ml) and supplemented with MEM Non-Essential Amino Acids Solution (NEAA) (Gibco Life Technologies, Gaithersburg, Md.).	Caco2 suggested: ATCC Cat# HTB-37, RRID:CVCL_0025)
   Biosynthesis inhibition and enzymatic treatment: HuH7.5 cells were treated in D-PBS/0.25% BSA with 46 miliunits heparinase III (Amsbio) for 1 hour at 37°C, washed and used in subsequent experiments.	HuH7.5 suggested: RRID:CVCL_7927)
   Software and Algorithms
   Sentences	Resources
   Data was analyzed using FlowJo vX.	FlowJo suggested: (FlowJo, RRID:SCR_008520)
   Statistical analyses were performed using GraphPad Prism 8 software and significance was set at *P< 0.05, **P<0.01***P<0.001****P<0.0001.	GraphPad Prism suggested: (GraphPad Prism, RRID:SCR_002798)

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   Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

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   Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

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   Results from TrialIdentifier: No clinical trial numbers were referenced.

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   Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

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   Results from JetFighter: We did not find any issues relating to colormaps.

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   Results from rtransparent:

   • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
   • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
   • No protocol registration statement was detected.

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3. 

   Version published to 10.1101/2020.08.18.255810 on bioRxiv

   Aug 18, 2020