Mechanism and inhibition of the papain‐like protease, PLpro, of SARS‐CoV‐2

  1. Theresa Klemm
  2. Gregor Ebert
  3. Dale J Calleja
  4. Cody C Allison
  5. Lachlan W Richardson
  6. Jonathan P Bernardini
  7. Bernadine GC Lu
  8. Nathan W Kuchel
  9. Christoph Grohmann
  10. Yuri Shibata
  11. Zhong Yan Gan
  12. James P Cooney
  13. Marcel Doerflinger
  14. Amanda E Au
  15. Timothy R Blackmore
  16. Gerbrand J van der Heden van Noort
  17. Paul P Geurink
  18. Huib Ovaa
  19. Janet Newman
  20. Alan Riboldi‐Tunnicliffe
  21. Peter E Czabotar
  22. Jeffrey P Mitchell
  23. Rebecca Feltham
  24. Bernhard C Lechtenberg
  25. Kym N Lowes
  26. Grant Dewson
  27. Marc Pellegrini
  28. Guillaume Lessene
  29. David Komander

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Abstract

No abstract available

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  1. Version published to 10.15252/embj.2020106275
  2. ScreenIT

    SciScore for 10.1101/2020.06.18.160614: (What is this?)

    Please note, not all rigor criteria are appropriate for all manuscripts.

    Table 1: Rigor

    Institutional Review Board Statementnot detected.
    Randomizationnot detected.
    Blindingnot detected.
    Power Analysisnot detected.
    Sex as a biological variablenot detected.

    Table 2: Resources

    Software and Algorithms
    SentencesResources
    Molecular Biology: Protein purification: PLpro activity assays: High throughput screening (HTS): Chemical synthesis of rac3k, rac3j and rac5c: The chemical synthesis, purification and characterisation of compounds are described in the Supplementary Information.
    Molecular Biology
    suggested: (Meharry Molecular Biology Core, RRID:SCR_012283)

    Results from OddPub: Thank you for sharing your data.

    Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

    Results from TrialIdentifier: No clinical trial numbers were referenced.

    Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

    Results from JetFighter: We did not find any issues relating to colormaps.

    Results from rtransparent:
    • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
    • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
    • No protocol registration statement was detected.

    About SciScore

    SciScore is an automated tool that is designed to assist expert reviewers by finding and presenting formulaic information scattered throughout a paper in a standard, easy to digest format. SciScore checks for the presence and correctness of RRIDs (research resource identifiers), and for rigor criteria such as sex and investigator blinding. For details on the theoretical underpinning of rigor criteria and the tools shown here, including references cited, please follow this link.

  3. Version published to 10.1101/2020.06.18.160614v1 on bioRxiv