Emergency response for evaluating SARS-CoV-2 immune status, seroprevalence and convalescent plasma in Argentina

Article activity feed

1. Version published to 10.1371/journal.ppat.1009161

   Jan 14, 2021
2. 

   ScreenIT

   Oct 26, 2020

   SciScore for 10.1101/2020.10.21.20216960: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Institutional Review Board Statement	IRB: The study was conducted in accordance with the Declaration of Helsinki, and was reviewed and approved by the Bioethics Committee of Fundación Instituto Leloir (HHS IRB # 00007572) (CBFIL Protocol #35). Consent: All participants provided written informed consent prior to the study.
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Sex as a biological variable	not detected.
   Cell Line Authentication	not detected.

   Table 2: Resources

   Antibodies
   Sentences	Resources
   Following a washing step with PBS-T, 100 μl of diluted horseradish peroxidase (HRP)-conjugated with goat anti-human IgM (Sigma), or with mouse anti-human IgG antibodies (BD pharmingen), …

   SciScore for 10.1101/2020.10.21.20216960: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Institutional Review Board Statement	IRB: The study was conducted in accordance with the Declaration of Helsinki, and was reviewed and approved by the Bioethics Committee of Fundación Instituto Leloir (HHS IRB # 00007572) (CBFIL Protocol #35). Consent: All participants provided written informed consent prior to the study.
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Sex as a biological variable	not detected.
   Cell Line Authentication	not detected.

   Table 2: Resources

   Antibodies
   Sentences	Resources
   Following a washing step with PBS-T, 100 μl of diluted horseradish peroxidase (HRP)-conjugated with goat anti-human IgM (Sigma), or with mouse anti-human IgG antibodies (BD pharmingen), was added to the wells and incubated for 30 min.	goat anti-human IgM suggested: None anti-human IgM suggested: None anti-human IgG suggested: None
   Experimental Models: Cell Lines
   Sentences	Resources
   For protein expression, FreeStyle293-F (293-F) cells or adherent HEK293-T (293-T) cells were maintained at 37°C with 8% CO2 and 5% CO2, respectively. 293-F cells were grown and transfected in Expi293™ expression medium and 293-T cells were grown in D-MEM high glucose supplemented with 10% Fetal Bovine Serum and Penicillin/Streptomycin 1% and transfected in Opti-MEM reduced serum media.	293-F suggested: RRID:CVCL_6642)
   Transfected 293-F suspension cultures were maintained at 32° and transfected 293-T cells were maintained at 37°C.	293-T suggested: None
   ACE2 and TMPRSS2 expressing 293T cells (293T-ACE2+TMPRSS2 clone F8-2), were used for these assays [31].	293T suggested: RRID:CVCL_YZ65)
   Software and Algorithms
   Sentences	Resources
   Enzyme-linked immunosorbent assays development: The ELISA protocol was adapted from previously established protocols used for Chagas disease by Laboratorio Lemos S.R.L. [42], and for SARS-CoV2 by the Krammer Laboratory [41].	Krammer Laboratory suggested: None
   Absolute inhibitory concentrations (absIC) values were calculated for all patient sera samples by modeling a 4-parameter logistic (4PL) regression with GraphPad Prism 8.	GraphPad Prism suggested: (GraphPad Prism, RRID:SCR_002798)

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   Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

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   Results from LimitationRecognizer: We detected the following sentences addressing limitations in the study:

   In this regard, an important limitation has been to obtain harmonized antibody titers information used in different clinical trials and therapeutic applications. By tittering more than 500 convalescent plasma samples, we showed a diversity of titers, which correlated with donor disease severity (Figure 5A). We provided a standardized protocol, widely distributed and used in Argentina for quantitative IgG measurements. A positive correlation between IgG quantitation, using COVIDAR, with neutralizing activity measured by a pseudotyped virus was shown (Figure 5C). This protocol has been useful to normalize quantifications at hospitals and heath institutions using plasma for compassionate therapies and for different clinical trials [22,23]. Although IgG titers correlated with neutralizing activities, the relevant open question is how they correlate with protection [38,39]. While the pandemic still progresses, scientists, public health workers, and policy makers are being challenged to create new strategies based on evidence and experiences in different parts of the world. A consensus has emerged that serological testing provides an essential tool in the pandemic response, nevertheless, serology testing strategies should be dynamic and adaptable to specific needs and resources available in different regions. Our work provides widely and freely available robust serology reagents, protocols, and data on humoral responses of SARS-CoV-2 infected individuals that hopefully helps findin...

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   Results from TrialIdentifier: No clinical trial numbers were referenced.

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   Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

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   Results from JetFighter: We did not find any issues relating to colormaps.

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   Results from rtransparent:

   • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
   • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
   • No protocol registration statement was detected.

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   Read the original source
3. 

   Version published to 10.1101/2020.10.21.20216960v1 on medRxiv

   Oct 23, 2020