Rapid SARS-CoV-2 testing in primary material based on a novel multiplex RT-LAMP assay
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SciScore for 10.1101/2020.06.18.20130377: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Table 2: Resources
No key resources detected.
Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: We detected the following sentences addressing limitations in the study:Since qPCR is the gold-standard this can be interpreted as a minor limitation in specificity. However, qPCR itself does not reach a sensitivity of 100% (21). Consequently, it is not clear yet whether these individuals were …
SciScore for 10.1101/2020.06.18.20130377: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Table 2: Resources
No key resources detected.
Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: We detected the following sentences addressing limitations in the study:Since qPCR is the gold-standard this can be interpreted as a minor limitation in specificity. However, qPCR itself does not reach a sensitivity of 100% (21). Consequently, it is not clear yet whether these individuals were truly negative or missed by the qPCR assay. CRISPR/ Cas12 (11) or Cas13a (13) based assays are another promising way to detect RNA in a PCR-independent manner. Comparison of this approach with LAMP demonstrates a surprisingly high sensitivity even of the colorimetric one step LAMP assay. Very recently, a novel protocol for Cas13a - called STOP (‘SHERLOCK Testing in One Pot’) - has been described (22). As this replaces the isothermal RPA reaction of the original SHERLOCK protocol by a LAMP reaction, the authors use a thermo-stable Cas13a enzyme to enable performing the entire reaction at the same temperature. Whilst being a very interesting approach, nonetheless, this comes with the difficulty the reaction tube has to be opened for the final lateral flow assay used for detection. In the real-world POC testing setting, this would require the establishment of a ‘pre-amp’ and ‘post-amp’ area to avoid cross-contamination, which may limit its use. Alternatively, lateral flow could be replaced by using a fluorescent probe together with an appropriate simple detection device. However, the highest Ct value resulting in a positive STOP assay is - at about 30 cycles - in a similar range compared to a recently described Cas12a-based method (DETECTR) (22). The LAMP assa...
Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
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