Performance of nucleocapsid and spike-based SARS-CoV-2 serologic assays

  1. Zahra Rikhtegaran Tehrani
  2. Saman Saadat
  3. Ebtehal Saleh
  4. Xin Ouyang
  5. Niel Constantine
  6. Anthony L. DeVico
  7. Anthony D. Harris
  8. George K. Lewis
  9. Shyam Kottilil
  10. Mohammad M. Sajadi

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  1. ScreenIT

    SciScore for 10.1101/2020.08.05.20168476: (What is this?)

    Please note, not all rigor criteria are appropriate for all manuscripts.

    Table 1: Rigor

    Institutional Review Board Statementnot detected.
    Randomizationnot detected.
    Blindingnot detected.
    Power Analysisnot detected.
    Sex as a biological variablenot detected.

    Table 2: Resources

    Antibodies
    SentencesResources
    In-house ELISAs: We developed three different ELISAs for the qualitative evaluation of the human serum or plasma for detection of IgG or IgA antibodies in human serum or plasma, targeting the SARS-CoV-2 trimer spike protein (IgG and IgA ELISA) and IgG antibodies targeting the SARS-CoV-2 nucleocapsid antigen (IgG ELISA).
    IgA
    suggested: None
    SARS-CoV-2 trimer spike protein (IgG
    suggested: None
    IgA ELISA) and IgG
    suggested: None
    SARS-CoV-2 nucleocapsid antigen (IgG
    suggested: None

    Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

    Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

    Results from TrialIdentifier: No clinical trial numbers were referenced.

    Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

    Results from JetFighter: We did not find any issues relating to colormaps.

    Results from rtransparent:
    • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
    • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
    • No protocol registration statement was detected.

    About SciScore

    SciScore is an automated tool that is designed to assist expert reviewers by finding and presenting formulaic information scattered throughout a paper in a standard, easy to digest format. SciScore checks for the presence and correctness of RRIDs (research resource identifiers), and for rigor criteria such as sex and investigator blinding. For details on the theoretical underpinning of rigor criteria and the tools shown here, including references cited, please follow this link.

  2. Version published to 10.1371/journal.pone.0237828
  3. Version published to 10.1101/2020.08.05.20168476v1 on medRxiv