Viral surface geometry shapes influenza and coronavirus spike evolution through antibody pressure
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Abstract
The evolution of circulating viruses is shaped by their need to evade antibody response, which mainly targets the viral spike. Because of the high density of spikes on the viral surface, not all antigenic sites are targeted equally by antibodies. We offer here a geometry-based approach to predict and rank the probability of surface residues of SARS spike (S protein) and influenza H1N1 spike (hemagglutinin) to acquire antibody-escaping mutations utilizing in-silico models of viral structure. We used coarse-grained MD simulations to estimate the on-rate (targeting) of an antibody model to surface residues of the spike protein. Analyzing publicly available sequences, we found that spike surface sequence diversity of the pre-pandemic seasonal influenza H1N1 and the sarbecovirus subgenus highly correlates with our model prediction of antibody targeting. In particular, we identified an antibody-targeting gradient, which matches a mutability gradient along the main axis of the spike. This identifies the role of viral surface geometry in shaping the evolution of circulating viruses. For the 2009 H1N1 and SARS-CoV-2 pandemics, a mutability gradient along the main axis of the spike was not observed. Our model further allowed us to identify key residues of the SARS-CoV-2 spike at which antibody escape mutations have now occurred. Therefore, it can inform of the likely functional role of observed mutations and predict at which residues antibody-escaping mutation might arise.
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SciScore for 10.1101/2020.10.20.347641: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
NIH rigor criteria are not applicable to paper type.Table 2: Resources
Software and Algorithms Sentences Resources For HA and S, solvent-accessible residues were identified using pymol script “findSurfaceResidues” (https://pymolwiki.org/index.php/FindSurfaceResidues), which identifies atoms with a solvent accessible area greater than or equal to 20 Ang2 (HA) and 15 Ang2 (S). pymolsuggested: (PyMOL, RRID:SCR_000305)The alignment of those sequences was done using ‘GONNET’ scoring matrix in Matlab. Matlabsuggested: (MATLAB, RRID:SCR_001622)Results from OddPub: Thank you for sharing your code.
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this …SciScore for 10.1101/2020.10.20.347641: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
NIH rigor criteria are not applicable to paper type.Table 2: Resources
Software and Algorithms Sentences Resources For HA and S, solvent-accessible residues were identified using pymol script “findSurfaceResidues” (https://pymolwiki.org/index.php/FindSurfaceResidues), which identifies atoms with a solvent accessible area greater than or equal to 20 Ang2 (HA) and 15 Ang2 (S). pymolsuggested: (PyMOL, RRID:SCR_000305)The alignment of those sequences was done using ‘GONNET’ scoring matrix in Matlab. Matlabsuggested: (MATLAB, RRID:SCR_001622)Results from OddPub: Thank you for sharing your code.
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
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