A booster dose of Delta × Omicron hybrid mRNA vaccine produced broadly neutralizing antibody against Omicron and other SARS-CoV-2 variants

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1. Version published to 10.1186/s12929-022-00830-1

   Jul 7, 2022
2. 

   ScreenIT

   Feb 3, 2022

   SciScore for 10.1101/2022.01.31.478406: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Ethics	IACUC: Ethics statement: All mouse works were conducted in accordance with the “Guideline for the Care and Use of Laboratory Animals” as defined by the Council of Agriculture, Taiwan and was approved by the Institutional Animal Care and Use Committee of Academia Sinica (protocol ID: 20-05-1471).
   Sex as a biological variable	not detected.
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Cell Line Authentication	not detected.

   Table 2: Resources

   Antibodies
   Sentences	Resources
   After washing, the cells were incubated with anti-RBD polyclonal antibody (1μg/tube) at 4°C for 30 minutes.	anti-RBD suggested: None
   The cells were then washed two times, followed by 30-minute incubation with …

   SciScore for 10.1101/2022.01.31.478406: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Ethics	IACUC: Ethics statement: All mouse works were conducted in accordance with the “Guideline for the Care and Use of Laboratory Animals” as defined by the Council of Agriculture, Taiwan and was approved by the Institutional Animal Care and Use Committee of Academia Sinica (protocol ID: 20-05-1471).
   Sex as a biological variable	not detected.
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Cell Line Authentication	not detected.

   Table 2: Resources

   Antibodies
   Sentences	Resources
   After washing, the cells were incubated with anti-RBD polyclonal antibody (1μg/tube) at 4°C for 30 minutes.	anti-RBD suggested: None
   The cells were then washed two times, followed by 30-minute incubation with PE-goat-anti-mouse IgG (H+L) antibody (Jackson ImmunoResearch, PA, USA) at 4°C.	PE-goat-anti-mouse IgG suggested: None
   Experimental Models: Cell Lines
   Sentences	Resources
   RBD expression and binding assay: The variant-specific RBD mRNA was transfected into 293T cells via lipofectamine (Invitrogen, MA, USA) and the variant-specific RBD-LNP was transfected by directly added.	293T suggested: KCB Cat# KCB 200744YJ, RRID:CVCL_0063)
   To test the ability of RBD binding to human ACE2 or mouse ACE2, 293T-hACE2 or 3T3-mACE2 cells were harvested and aliquoted into FACS tubes at 5×105 cells/tube.	3T3-mACE2 suggested: None
   The mixtures were then added to pre-seeded 293T-hACE2 cells and incubated for 3 days.	293T-hACE2 suggested: None
   Experimental Models: Organisms/Strains
   Sentences	Resources
   Animals: BALB/c mice were purchased from the National Laboratory Animal Center (Taipei, Taiwan) and maintained in a specific pathogen-free environment in the animal facilities of the Institute of Biomedical Sciences, Academia Sinica.	BALB/c suggested: RRID:IMSR_ORNL:BALB/cRl)
   Software and Algorithms
   Sentences	Resources
   The cells were washed twice and resuspended in 300 μl of staining buffer containing 7-AAD (Biolegend, CA, USA) for flow cytometry analysis (Thermo Fisher Attune NxT - 14 color analyzer, Thermo Fisher Attune NxT software v2.2, FlowJo 10.6.1).	Thermo Fisher Attune NxT suggested: (Attune Nxt Nxt, RRID:SCR_019590) FlowJo suggested: (FlowJo, RRID:SCR_008520)
   The 50% neutralization titer (NT50) was calculated by nonlinear regression using Prism software version 8.1.0 (	Prism suggested: (PRISM, RRID:SCR_005375)
   GraphPad Software Inc.)	GraphPad suggested: (GraphPad Prism, RRID:SCR_002798)

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   Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

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   Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

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   Results from TrialIdentifier: No clinical trial numbers were referenced.

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   Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

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   Results from JetFighter: Please consider improving the rainbow (“jet”) colormap(s) used on page 14. At least one figure is not accessible to readers with colorblindness and/or is not true to the data, i.e. not perceptually uniform.

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   Results from rtransparent:

   • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
   • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
   • No protocol registration statement was detected.

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   Results from scite Reference Check: We found no unreliable references.

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3. 

   Version published to 10.1101/2022.01.31.478406 on bioRxiv

   Jan 31, 2022