Increased expression of ACE2, the SARS-CoV-2 entry receptor, in alveolar and bronchial epithelium of smokers and COPD subjects
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SciScore for 10.1101/2020.05.27.20114298: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement Consent: Written informed consent was obtained from all subjects.
IRB: This study was approved by the medical ethical committees of the Ghent University Hospital (2011/0114; 2016/0132; 2019/0537) and the University Hospital Gasthuisberg Leuven (S51577)Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Table 2: Resources
Antibodies Sentences Resources After antigen retrieval with citrate buffer (Scytek), the slides were incubated with anti-ACE2 antibody (polyclonal rabbit-anti-human, Abcam ab15248). anti-ACE2suggested: Nonerabbit-anti-human,suggested: NoneSoftware and Algorithms Sentences Resources Statistic… SciScore for 10.1101/2020.05.27.20114298: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement Consent: Written informed consent was obtained from all subjects.
IRB: This study was approved by the medical ethical committees of the Ghent University Hospital (2011/0114; 2016/0132; 2019/0537) and the University Hospital Gasthuisberg Leuven (S51577)Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Table 2: Resources
Antibodies Sentences Resources After antigen retrieval with citrate buffer (Scytek), the slides were incubated with anti-ACE2 antibody (polyclonal rabbit-anti-human, Abcam ab15248). anti-ACE2suggested: Nonerabbit-anti-human,suggested: NoneSoftware and Algorithms Sentences Resources Statistical analysis: Statistical analysis was performed with Sigma Stat software (SPSS 26.0, Chicago, IL, USA) and R3.5.1, using Student’s t-test, Kruskal-Wallis and Mann-Whitney U test, Fisher’s exact test, Spearman’s Rank correlations, and multivariate linear regression analyses. Sigma Statsuggested: NoneSPSSsuggested: (SPSS, RRID:SCR_002865)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: We detected the following sentences addressing limitations in the study:We measured mRNA expression of ACE2 and TMPRSS2 in lung tissue samples from 120 never smokers and smokers with and without airflow limitation and demonstrate higher ACE2 mRNA levels in current smokers and patients with moderate (GOLD II) and severe to very severe COPD (GOLD III-IV). Importantly, ACE2 mRNA expression shows an inverse correlation with physiological parameters of airway obstruction and emphysema. Linear regression analysis confirms that smoking and COPD are associated with increased ACE2 mRNA expression, independent of covariables such as age, gender, comorbidities, and medication use. The association between COPD and ACE2 mRNA seems to be partly driven by smoking as the effect size of the association between COPD and ACE2 mRNA decreased after adjusting for smoking. The remaining statistically significant association between COPD and ACE2 mRNA might be explained by other factors such as aggravated pulmonary and systemic inflammation, previous exacerbations or genetic predisposition. Interestingly, a recent large meta-analysis of transcriptomic data confirms the increased ACE2 mRNA expression in lung tissue of smokers and patients with COPD [28]. TMPRSS2 mRNA expression is only significantly higher in patients with (very) severe COPD. However, there is a significant correlation between TMPRSS mRNA and ACE2 mRNA expression levels, even in sensitivity analyses omitting (very) severe COPD patients from the analysis. Our data suggest that both ACE2 and TMPRSS2 are ex...
Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
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- No protocol registration statement was detected.
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