Functional monocytic myeloid-derived suppressor cells increase in blood but not airways and predict COVID-19 severity
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SciScore for 10.1101/2020.09.08.20190272: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement Consent: Written informed consent was obtained from all patients and controls. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Table 2: Resources
Antibodies Sentences Resources Flow cytometry: Cells were stained using Live/Dead Blue (Invitrogen), incubated with human FcR blocking reagent (Miltenyi Biotec) and stained with antibodies against the following surface proteins: CD1c (AD5-8E7; Miltenyi Biotec), CD3 (SK7; BD), CD11c (B-Ly6; BD) CD3suggested: NoneCD11csuggested: NoneDetection was performed with a goat anti-human IgG HRP-conjugated secondary antibody (clone G18-145 from BD Biosciences) … SciScore for 10.1101/2020.09.08.20190272: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement Consent: Written informed consent was obtained from all patients and controls. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Table 2: Resources
Antibodies Sentences Resources Flow cytometry: Cells were stained using Live/Dead Blue (Invitrogen), incubated with human FcR blocking reagent (Miltenyi Biotec) and stained with antibodies against the following surface proteins: CD1c (AD5-8E7; Miltenyi Biotec), CD3 (SK7; BD), CD11c (B-Ly6; BD) CD3suggested: NoneCD11csuggested: NoneDetection was performed with a goat anti-human IgG HRP-conjugated secondary antibody (clone G18-145 from BD Biosciences) followed by incubation with TMB substrate (BioLegend; cat# 421101) and stopped with a 1M solution of H2SO4. anti-human IgGsuggested: NoneSoftware and Algorithms Sentences Resources Data were analysed using FlowJo software 10.5.3 (TreeStar). FlowJosuggested: (FlowJo, RRID:SCR_008520)Statistical analysis: Data analysis was performed in RStudio version 1.2 (RStudio Inc., Boston, MA), RStudiosuggested: (RStudio, RRID:SCR_000432)GraphPad Prism version 8.0 (GraphPad Software Inc. GraphPad Prismsuggested: (GraphPad Prism, RRID:SCR_002798)GraphPadsuggested: (GraphPad Prism, RRID:SCR_002798)San Diego, CA) and Microsoft Excel (Microsoft Corp., Redmond, WA). Microsoft Excelsuggested: (Microsoft Excel, RRID:SCR_016137)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: Please consider improving the rainbow (“jet”) colormap(s) used on page 36. At least one figure is not accessible to readers with colorblindness and/or is not true to the data, i.e. not perceptually uniform.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
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