Neutralizing antibody activity against SARS-CoV-2 variants in gestational age–matched mother-infant dyads after infection or vaccination
This article has been Reviewed by the following groups
Listed in
- Evaluated articles (ScreenIT)
Abstract
No abstract available
Article activity feed
-
-
SciScore for 10.1101/2021.12.09.21267557: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Ethics Field Sample Permit: Maternal blood and infant cord blood used were collected at delivery in an EDTA collection tube and were processed within 24 hours.
IRB: Study approval: This study was approved by the institutional review board of the UCSF (IRB# 20-32077), Santa Clara Valley Medical Center (IRB# 20-021), Oregon Health and Sciences University (IRB# STUDY00021569) and Marshall University (IRB# 1662248-1).
Consent: Written informed consent was obtained from all participants.Sex as a biological variable Thirty vaccinated and 30 infected pregnant women were chosen from these two cohorts to be matched on the approximate gestational age of first vaccine dose and the earliest confirmed SARS-CoV-2 … SciScore for 10.1101/2021.12.09.21267557: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Ethics Field Sample Permit: Maternal blood and infant cord blood used were collected at delivery in an EDTA collection tube and were processed within 24 hours.
IRB: Study approval: This study was approved by the institutional review board of the UCSF (IRB# 20-32077), Santa Clara Valley Medical Center (IRB# 20-021), Oregon Health and Sciences University (IRB# STUDY00021569) and Marshall University (IRB# 1662248-1).
Consent: Written informed consent was obtained from all participants.Sex as a biological variable Thirty vaccinated and 30 infected pregnant women were chosen from these two cohorts to be matched on the approximate gestational age of first vaccine dose and the earliest confirmed SARS-CoV-2 test. Randomization not detected. Blinding not detected. Power Analysis not detected. Cell Line Authentication not detected. Table 2: Resources
Antibodies Sentences Resources Correlations between NT50 titers and IgG antibody titers were analyzed using Pearson’s rank test. IgGsuggested: NoneExperimental Models: Cell Lines Sentences Resources Twenty-four hours before administration of virion, 2.5×104 Calu-6 hACE2 cells were plated per well of a 96-well plate in 200 μL of complete DMEM. Calu-6 hACE2suggested: NoneThe SARS-CoV-2 spike pseudotyped virions harvested from the supernatant of the 293T cells were assayed for titration and then aliquots mixed for 30 minutes with heat-inactivated plasma samples. 293Tsuggested: NoneSoftware and Algorithms Sentences Resources Results were analyzed by Prism software version 9 (Graph Pad). Prismsuggested: (PRISM, RRID:SCR_005375)IgG Antibody Measurement: Antibodies to SARS-CoV-2 IgG was measured using the Pylon 3D automated immunoassay system (ET Healthcare, Palo Alto, California) as described previously (63). ET Healthcaresuggested: NoneHeatmaps were created in R studio (pheatmap) and GraphPad Prism. GraphPad Prismsuggested: (GraphPad Prism, RRID:SCR_002798)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: We detected the following sentences addressing limitations in the study:There are several limitations and strength of our study. First, we have few patients that were exposed in the first trimester. However, this is balanced against a significant strength of our study in that the participants were matched by timing of vaccine or live viral exposure during pregnancy, allowing direct comparison of vaccine-induced and natural immunity in pregnancy and different timepoints in pregnancy. Second, we do not have sequencing data for the natural infection cohort, and thus do not know which strains caused the infection. However, all infected patients tested positive before January 2021, prior to broad circulation of the Alpha and Delta variants in the United States (60, 61). Presumably, individuals infected with the Delta variant would have higher specific nAb activity against this strain and may also have differing responses to the other variants. Future studies would be helpful to understand the immunogenicity evoked by the Delta, Mu, and other variants in pregnancy. Third, detailed antibody characterization, such as IgG subtyping or fucosylation state, is needed to understand the role of these factors in the dynamics of maternal-fetal antibody transfer. This should be addressed in future studies. In conclusion, vaccination in pregnancy is highly effective in generating nAbs against all strains of SARS-CoV-2 tested, although activities against the Kappa, Delta, and Mu variants are reduced. Vaccine-induced neutralizing activity is comparable to natural im...
Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
Results from scite Reference Check: We found no unreliable references.
-
