A functionally distinct neutrophil landscape in severe COVID-19 reveals opportunities for adjunctive therapies
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SciScore for 10.1101/2021.06.07.21258484: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Ethics Consent: Study design: Written informed consent was obtained from all study participants or most appropriate surrogate decision maker for patients who were unable to provide consent.
IRB: This study was approved by the conjoint health research ethics board of the University of Calgary and Alberta Health Services (Sex as a biological variable not detected. Randomization To visualize the neutrophil metacluster landscape, tSNE dimensionality reduction was performed on 2,500 randomly selected events from each sample using a perplexity of 70 for 5000 iterations. Blinding not detected. Power Analysis not detected. Table 2: Resources
Experimental Models: Organisms/Strains Sentences Resources Between April 1 … SciScore for 10.1101/2021.06.07.21258484: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Ethics Consent: Study design: Written informed consent was obtained from all study participants or most appropriate surrogate decision maker for patients who were unable to provide consent.
IRB: This study was approved by the conjoint health research ethics board of the University of Calgary and Alberta Health Services (Sex as a biological variable not detected. Randomization To visualize the neutrophil metacluster landscape, tSNE dimensionality reduction was performed on 2,500 randomly selected events from each sample using a perplexity of 70 for 5000 iterations. Blinding not detected. Power Analysis not detected. Table 2: Resources
Experimental Models: Organisms/Strains Sentences Resources Between April 1 2020 and March 30 2021, consecutive patients were screened for the following inclusion criteria: adult patients (>18 years of age) with an index admission to one of four multi-system intensive care units in Calgary, AB, Canada, with a diagnosis of ARDS based on the Berlin Criteria (30), associated with a diagnosis of SARS-CoV-2 infection (based on clinical laboratory qPCR assay from nasopharyngeal swab or endotracheal tube aspirate) or bacterial pneumonia (based on standard clinical and microbiologic criteria). ABsuggested: RRID:BDSC_203)Software and Algorithms Sentences Resources For mass cytometry data analysis, FCS files were imported into Cytobank (Cytobank.org) for data visualization and manual gating. Cytobanksuggested: (Cytobank, RRID:SCR_014043)Single-cell events were then exported from CytoBank as FCS files and loaded into the CATALYST package (32) in R (R Development Core Team) CATALYSTsuggested: (CATALYST, RRID:SCR_017127)R Development Coresuggested: (R Project for Statistical Computing, RRID:SCR_001905)For neutrophil clustering and analysis, CD45+CD66b+ neutrophils were analyzed by FlowSOM (33) and a Consensus Clustering method (34) was employed on all neutrophil single-cell events within CATALYST based on the expression of neutrophil markers (see Fig 1C). FlowSOMsuggested: (FlowSOM, RRID:SCR_016899)Data visualization was generated using the built-in functions of CATALYST and the ggplot2 package in R. ggplot2suggested: (ggplot2, RRID:SCR_014601)Neutrophils were gated based on their forward and side scatter profiles, and bacterial phagocytosis was measured by as the percentage of neutrophils positive for GFP+ bacteria using FlowJo software (FloJo, BD Biosciences). FlowJosuggested: (FlowJo, RRID:SCR_008520)Principal component analysis of neutrophil markers expression between patient groups was performed using the procomp function and VEGAN package in R, and plotted using ggplot2. VEGANsuggested: (vegan, RRID:SCR_011950)Statistical analysis and graphic generation were performed using Graphpad Prism (GraphPad Software) and R (R Development Core Team). Graphpad Prismsuggested: (GraphPad Prism, RRID:SCR_002798)GraphPadsuggested: (GraphPad Prism, RRID:SCR_002798)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: We found the following clinical trial numbers in your paper:
Identifier Status Title NCT04402957 Active, not recruiting LSALT Peptide vs. Placebo to Prevent ARDS and Acute Kidney I… Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
Results from scite Reference Check: We found no unreliable references.
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