Airway antibodies emerge according to COVID-19 severity and wane rapidly but reappear after SARS-CoV-2 vaccination
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SciScore for 10.1101/2020.11.25.20238592: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement Consent: Written informed consent was obtained from all patients and controls. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Table 2: Resources
Antibodies Sentences Resources Detection was performed with mouse and goat anti-human IgG or IgA HRP-conjugated secondary antibodies (clone G18-145 from BD Biosciences and polyclonal from ThermoFisher, respectively) followed by incubation with TMB substrate (BioLegend) which was stopped with a 1M solution of sulfuric acid. anti-human IgGsuggested: NoneIgA HRP-conjugated secondary antibodiessuggested: NoneSoftware and Algorithms Sentences Resources Duplicate … SciScore for 10.1101/2020.11.25.20238592: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement Consent: Written informed consent was obtained from all patients and controls. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Table 2: Resources
Antibodies Sentences Resources Detection was performed with mouse and goat anti-human IgG or IgA HRP-conjugated secondary antibodies (clone G18-145 from BD Biosciences and polyclonal from ThermoFisher, respectively) followed by incubation with TMB substrate (BioLegend) which was stopped with a 1M solution of sulfuric acid. anti-human IgGsuggested: NoneIgA HRP-conjugated secondary antibodiessuggested: NoneSoftware and Algorithms Sentences Resources Duplicate 7-point serial dilutions were initially performed for measuring plasma IgG against RBD during acute disease and the half maximal effective concentration (EC50) was calculated using GraphPad Prism 9. GraphPad Prismsuggested: (GraphPad Prism, RRID:SCR_002798)Stained cells from airway samples were acquired using a BD LSRFortessa while stained PBMC were acquired using a BD FACSAria Fusion both interfaced with the BD FACSDiva Software. BD FACSDivasuggested: (BD FACSDiva Software, RRID:SCR_001456)Results were analyzed using BD FlowJo version 10. FlowJosuggested: (FlowJo, RRID:SCR_008520)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
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