Autoantibodies stabilize neutrophil extracellular traps in COVID-19
This article has been Reviewed by the following groups
Listed in
- Evaluated articles (ScreenIT)
Abstract
No abstract available
Article activity feed
-
-
-
SciScore for 10.1101/2021.03.31.21254692: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable The 48 controls included 40 females and 8 males, with a mean age and standard deviation of 38 ± 10. Table 2: Resources
Antibodies Sentences Resources For protein staining, fixed cells were incubated with either control or COVID serum (diluted to 10%) and polyclonal antibody to neutrophil elastase (Abcam Ab21595) diluted 1:100 in blocking buffer for 1 hour at 4°C, followed by fluorochrome-conjugated DyLight594 anti-human IgG (Thermo Fisher) and FITC-conjugated secondary (Southern Biotech) for 1 hour at 4°C. neutrophil elastasesuggested: Noneanti-human IgGSciScore for 10.1101/2021.03.31.21254692: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable The 48 controls included 40 females and 8 males, with a mean age and standard deviation of 38 ± 10. Table 2: Resources
Antibodies Sentences Resources For protein staining, fixed cells were incubated with either control or COVID serum (diluted to 10%) and polyclonal antibody to neutrophil elastase (Abcam Ab21595) diluted 1:100 in blocking buffer for 1 hour at 4°C, followed by fluorochrome-conjugated DyLight594 anti-human IgG (Thermo Fisher) and FITC-conjugated secondary (Southern Biotech) for 1 hour at 4°C. neutrophil elastasesuggested: Noneanti-human IgGsuggested: NoneSoftware and Algorithms Sentences Resources Data analysis was with GraphPad Prism software version 8. GraphPad Prismsuggested: (GraphPad Prism, RRID:SCR_002798)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
-