Distinct antibody repertoires against endemic human coronaviruses in children and adults
This article has been Reviewed by the following groups
Listed in
- Evaluated articles (ScreenIT)
Abstract
No abstract available
Article activity feed
-
-
-
SciScore for 10.1101/2020.06.21.163394: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement IRB: The human subject research described here had been approved by the institutional research ethics boards of Sidra Medicine, Qatar Biobank, INSERM, and Erasme Hospital. Randomization For the purpose of this study, specimen from male Qatari nationals 19 to 66 years of age (Supplementary Table S1) were selected from a larger blood donor cohort including 5983 individuals, and then age-matched male donors with other nationalities were randomly selected (Supplementary Figure S1). Blinding not detected. Power Analysis not detected. Sex as a biological variable Study design and samples: We performed a retrospective analysis of deidentified or coded plasma and serum samples … SciScore for 10.1101/2020.06.21.163394: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement IRB: The human subject research described here had been approved by the institutional research ethics boards of Sidra Medicine, Qatar Biobank, INSERM, and Erasme Hospital. Randomization For the purpose of this study, specimen from male Qatari nationals 19 to 66 years of age (Supplementary Table S1) were selected from a larger blood donor cohort including 5983 individuals, and then age-matched male donors with other nationalities were randomly selected (Supplementary Figure S1). Blinding not detected. Power Analysis not detected. Sex as a biological variable Study design and samples: We performed a retrospective analysis of deidentified or coded plasma and serum samples collected from three different human cohorts, namely: i) 400 healthy male adult blood donors (ABD) of a blood bank in Qatar with diverse ethnic background and nationality (Supplementary Figure S1A); ii) 800 adult male and female Qatari nationals and long-term residents of Qatar who are participating in a national cohort study— the Qatar Biobank (QBB)—and who represent the general local population in the State of Qatar [32]; and iii) 231 pediatric subjects with Qatari nationality who were admitted to, or visited outpatient clinics of, Sidra Medicine. Table 2: Resources
Software and Algorithms Sentences Resources Software: Open source Python modules with in-house scripts were used to test for associations (statsmodel v0.11), to filter differentially enrichment peptides and to perform different statistical tests (sklearn v0.23, scipy v1.14.1). Pythonsuggested: (IPython, RRID:SCR_001658)scipysuggested: (SciPy, RRID:SCR_008058)Multiple sequence alignments were done using the MAFFT [73, 74] via EMBL-EBI’s web services and Java Alignment Viewer (Jalview) for visualization [75]. MAFFTsuggested: (MAFFT, RRID:SCR_011811)Jalviewsuggested: (Jalview, RRID:SCR_006459)Protein structures graphics were generated using PyMOL (Schrödinger). PyMOLsuggested: (PyMOL, RRID:SCR_000305)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
-