Cytokine profile in plasma of severe COVID-19 does not differ from ARDS and sepsis
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SciScore for 10.1101/2020.05.15.20103549: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement Consent: All patients or their surrogates gave written informed consent to participate in the Stanford ICU Biobank. Randomization Patients who were enrolled in randomized clinical trials that required blood draws were excluded. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Table 2: Resources
Software and Algorithms Sentences Resources To assess cytokine response to infection in patients with a normal immune system (similar to the COVID population), the ARDS and sepsis patients were therefore enriched for normal baseline immune system (e.g. no metastatic cancer, bone marrow transplant, or high dose steroids) in comparison to … SciScore for 10.1101/2020.05.15.20103549: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement Consent: All patients or their surrogates gave written informed consent to participate in the Stanford ICU Biobank. Randomization Patients who were enrolled in randomized clinical trials that required blood draws were excluded. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Table 2: Resources
Software and Algorithms Sentences Resources To assess cytokine response to infection in patients with a normal immune system (similar to the COVID population), the ARDS and sepsis patients were therefore enriched for normal baseline immune system (e.g. no metastatic cancer, bone marrow transplant, or high dose steroids) in comparison to the Biobank as a whole. Biobanksuggested: (HIV Biobank, RRID:SCR_004691)Statistics: All statistical analyses were performed using the open source statistical software R (https://www.r-project.org). https://www.r-project.orgsuggested: (R Project for Statistical Computing, RRID:SCR_001905)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: We detected the following sentences addressing limitations in the study:The most important limitation of this study is our small sample size. Even in our predetermined analysis of six cytokines strongly associated with cytokine storm, we lack power to detect minor differences between groups. The exploratory analyses of an additional 70 cytokines is similarly limited, but is provided as a reference for the field. In addition, we do not have measurements of cytokines over time, but only near the point of enrollment. Finally, as discussed above, we report cytokine levels by MFI per recommended Luminex analysis methods,10 precluding direct comparison of our values to previously published data that report cytokine levels in pg/mL. Despite the above limitations, it is clear that in this cohort, inflammatory cytokines are not dramatically higher in severe COVID-19 patients than in other critically ill patients. Broad testing of immunosuppressive therapies in unselected COVID-19 patients may therefore face the same fate as trials of immunosuppression in unselected ARDS and sepsis patients: inconclusive results despite decades of effort, and the uncomfortable understanding that while some patients may have been helped, others may have been harmed. Indeed, given the duration of mechanical ventilation and attendant high rates of nosocomial infection in severe COVID-19 patients, these therapies have potential for harm. Further research is needed to characterize the spectrum of the immune response in COVID-19, and to identify which patients are most likely to...
Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
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- No protocol registration statement was detected.
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