Lack of Evidence of Angiotensin-Converting Enzyme 2 Expression and Replicative Infection by SARS-CoV-2 in Human Endothelial Cells
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SciScore for 10.1101/2020.12.02.391664: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Experimental Models: Cell Lines Sentences Resources HUVEC were plated at a density of 10,000 cells/96-well plate in EGM-2 supplemented with recombinant human TNF-α (300-01A, PeproTech EC Ltd), IL1-β (200-01B, PeproTech EC Ltd), IL8 (72aa, monocytes derived, 200-08M, PeproTech EC Ltd) and IL-6/IL-6R Alpha Protein Chimera (8954-SR, R&D Biotechne) at 0, 0.01, 0.1 or 1.0 ng/ml for 4 or 24 hours. HUVECsuggested: RRID:CVCL_A4BD)Software and Algorithms Sentences Resources Whole-transcriptome … SciScore for 10.1101/2020.12.02.391664: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Experimental Models: Cell Lines Sentences Resources HUVEC were plated at a density of 10,000 cells/96-well plate in EGM-2 supplemented with recombinant human TNF-α (300-01A, PeproTech EC Ltd), IL1-β (200-01B, PeproTech EC Ltd), IL8 (72aa, monocytes derived, 200-08M, PeproTech EC Ltd) and IL-6/IL-6R Alpha Protein Chimera (8954-SR, R&D Biotechne) at 0, 0.01, 0.1 or 1.0 ng/ml for 4 or 24 hours. HUVECsuggested: RRID:CVCL_A4BD)Software and Algorithms Sentences Resources Whole-transcriptome analysis with total RNA sequencing (RNA-Seq): RNA-seq data files with gene quantifications for primary human epithelial and endothelial cells were downloaded from the ENCODE database (www.encodeproject.org)6, using Bioconductor package “ENCODEExplorer” and R. Bioconductorsuggested: (Bioconductor, RRID:SCR_006442)The data were processed using Seurat package (version: 3.1.1) in R. Seuratsuggested: (SEURAT, RRID:SCR_007322)ChIP-seq reads were mapped to the human reference genome GRCh37/hg19. ChIP-seqsuggested: (ChIP-seq, RRID:SCR_001237)Tracks were visualized using the UCSC Genome Browser database (https://genome.ucsc.edu). UCSC Genome Browsersuggested: (UCSC Genome Browser, RRID:SCR_005780)HUVEC chromatin-state discovery and genome annotation was obtained using ChromHMM7 from ENCODE. ENCODEsuggested: (Encode, RRID:SCR_015482)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
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