Lack of Evidence of Angiotensin-Converting Enzyme 2 Expression and Replicative Infection by SARS-CoV-2 in Human Endothelial Cells

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1. Version published to 10.1161/circulationaha.120.052824

   Feb 23, 2021
2. 

   ScreenIT

   Dec 3, 2020

   SciScore for 10.1101/2020.12.02.391664: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Institutional Review Board Statement	not detected.
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Sex as a biological variable	not detected.
   Cell Line Authentication	not detected.

   Table 2: Resources

   Experimental Models: Cell Lines
   Sentences	Resources
   HUVEC were plated at a density of 10,000 cells/96-well plate in EGM-2 supplemented with recombinant human TNF-α (300-01A, PeproTech EC Ltd), IL1-β (200-01B, PeproTech EC Ltd), IL8 (72aa, monocytes derived, 200-08M, PeproTech EC Ltd) and IL-6/IL-6R Alpha Protein Chimera (8954-SR, R&D Biotechne) at 0, 0.01, 0.1 or 1.0 ng/ml for 4 or 24 hours.	HUVEC suggested: RRID:CVCL_A4BD)
   Software and Algorithms
   Sentences	Resources
   Whole-transcriptome …

   SciScore for 10.1101/2020.12.02.391664: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Institutional Review Board Statement	not detected.
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Sex as a biological variable	not detected.
   Cell Line Authentication	not detected.

   Table 2: Resources

   Experimental Models: Cell Lines
   Sentences	Resources
   HUVEC were plated at a density of 10,000 cells/96-well plate in EGM-2 supplemented with recombinant human TNF-α (300-01A, PeproTech EC Ltd), IL1-β (200-01B, PeproTech EC Ltd), IL8 (72aa, monocytes derived, 200-08M, PeproTech EC Ltd) and IL-6/IL-6R Alpha Protein Chimera (8954-SR, R&D Biotechne) at 0, 0.01, 0.1 or 1.0 ng/ml for 4 or 24 hours.	HUVEC suggested: RRID:CVCL_A4BD)
   Software and Algorithms
   Sentences	Resources
   Whole-transcriptome analysis with total RNA sequencing (RNA-Seq): RNA-seq data files with gene quantifications for primary human epithelial and endothelial cells were downloaded from the ENCODE database (www.encodeproject.org)6, using Bioconductor package “ENCODEExplorer” and R.	Bioconductor suggested: (Bioconductor, RRID:SCR_006442)
   The data were processed using Seurat package (version: 3.1.1) in R.	Seurat suggested: (SEURAT, RRID:SCR_007322)
   ChIP-seq reads were mapped to the human reference genome GRCh37/hg19.	ChIP-seq suggested: (ChIP-seq, RRID:SCR_001237)
   Tracks were visualized using the UCSC Genome Browser database (https://genome.ucsc.edu).	UCSC Genome Browser suggested: (UCSC Genome Browser, RRID:SCR_005780)
   HUVEC chromatin-state discovery and genome annotation was obtained using ChromHMM7 from ENCODE.	ENCODE suggested: (Encode, RRID:SCR_015482)

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   Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

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   Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

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   Results from TrialIdentifier: No clinical trial numbers were referenced.

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   Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

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   Results from JetFighter: We did not find any issues relating to colormaps.

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   Results from rtransparent:

   • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
   • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
   • No protocol registration statement was detected.

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   Read the original source
3. 

   Version published to 10.1101/2020.12.02.391664v1 on bioRxiv

   Dec 2, 2020