Viral RNA Load and Infectivity of SARS-CoV-2 in Paired Respiratory and Oral Specimens from Symptomatic, Asymptomatic, or Postsymptomatic Individuals

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1. Version published to 10.1128/spectrum.02264-21

   Jun 29, 2022
2. 

   ScreenIT

   Nov 16, 2021

   SciScore for 10.1101/2021.11.13.21266305: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Ethics	IRB: CMC) Institutional Review Board approval 0420EP. Consent: All patients agreed to participate and provided verbal consent prior to specimen collection.
   Sex as a biological variable	not detected.
   Randomization	not detected.
   Blinding	Cell cultures with no CPE were frozen, thawed, and subjected to two additional blind passages/inoculations in Vero E6/TMPRSS2 cell cultures.
   Power Analysis	not detected.
   Cell Line Authentication	not detected.

   Table 2: Resources

   Experimental Models: Cell Lines
   Sentences	Resources
   For this, twenty-four well plates were seeded with ∼75,000 Vero E6/TMPRSS2 cells per well 24 h prior to sample inoculation.	Vero E6/TMPRSS2 suggested: None
   Software and Algorithms
   Sentences	Resources
   The …

   SciScore for 10.1101/2021.11.13.21266305: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Ethics	IRB: CMC) Institutional Review Board approval 0420EP. Consent: All patients agreed to participate and provided verbal consent prior to specimen collection.
   Sex as a biological variable	not detected.
   Randomization	not detected.
   Blinding	Cell cultures with no CPE were frozen, thawed, and subjected to two additional blind passages/inoculations in Vero E6/TMPRSS2 cell cultures.
   Power Analysis	not detected.
   Cell Line Authentication	not detected.

   Table 2: Resources

   Experimental Models: Cell Lines
   Sentences	Resources
   For this, twenty-four well plates were seeded with ∼75,000 Vero E6/TMPRSS2 cells per well 24 h prior to sample inoculation.	Vero E6/TMPRSS2 suggested: None
   Software and Algorithms
   Sentences	Resources
   The Thermo Fisher TaqPath COVID-19	Thermo Fisher TaqPath suggested: None
   Statistical analysis: Diagnostic sensitivity and result concordance across specimens and platforms were calculated with Microsoft Excel (Microsoft Office Professional Plus 2019)	Microsoft Excel suggested: (Microsoft Excel, RRID:SCR_016137)
   GraphPad Prism (version 9.0.2 for Windows, GraphPad Software, San Diego, CA, USA, www.graphpad.com) was used to perform testing for normal distribution of cycle threshold (Ct) values with Shapiro-Wilk tests, comparison of Ct values using Wilcoxon matched-pairs signed rank tests or Mann-Whitney tests for unpaired comparisons, and to generate plots.	GraphPad Prism suggested: (GraphPad Prism, RRID:SCR_002798) GraphPad suggested: (GraphPad Prism, RRID:SCR_002798)

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   Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

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   Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

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   Results from TrialIdentifier: No clinical trial numbers were referenced.

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   Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

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   Results from JetFighter: We did not find any issues relating to colormaps.

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   Results from rtransparent:

   • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
   • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
   • Thank you for including a protocol registration statement.

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   Results from scite Reference Check: We found no unreliable references.

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   Read the original source
3. 

   Version published to 10.1101/2021.11.13.21266305v1 on medRxiv

   Nov 14, 2021