Age-Dependent Reduction in Neutralization against Alpha and Beta Variants of BNT162b2 SARS-CoV-2 Vaccine-Induced Immunity

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1. Version published to 10.1128/spectrum.00561-21

   Dec 22, 2021
2. 

   ScreenIT

   Jun 3, 2021

   SciScore for 10.1101/2021.05.26.21257884: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Ethics	Field Sample Permit: VeroE6/TMPRSS2 cells (JCRB1819) were purchased from the Japanese Collection of Research Bioresources (JCRB) Cell Bank (Osaka, Japan). IRB: Ethics approval: This study was performed in accordance with the Declaration of Helsinki and was approved by the ethical review board of the University of Toyama (approval No.: R2019167). Consent: Written informed consent was obtained from all participants.
   Sex as a biological variable	not detected.
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Cell Line Authentication	not detected.

   Table 2: Resources

   Experimental Models: Cell Lines
   Sentences	Resources
   Briefly, 293T cells were transfected with the above expression …

   SciScore for 10.1101/2021.05.26.21257884: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Ethics	Field Sample Permit: VeroE6/TMPRSS2 cells (JCRB1819) were purchased from the Japanese Collection of Research Bioresources (JCRB) Cell Bank (Osaka, Japan). IRB: Ethics approval: This study was performed in accordance with the Declaration of Helsinki and was approved by the ethical review board of the University of Toyama (approval No.: R2019167). Consent: Written informed consent was obtained from all participants.
   Sex as a biological variable	not detected.
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Cell Line Authentication	not detected.

   Table 2: Resources

   Experimental Models: Cell Lines
   Sentences	Resources
   Briefly, 293T cells were transfected with the above expression vectors.	293T suggested: CCLV Cat# CCLV-RIE 1018, RRID:CVCL_0063)
   VeroE6/TMPRSS2 cells (JCRB1819) were purchased from the Japanese Collection of Research Bioresources (JCRB) Cell Bank (Osaka, Japan).	VeroE6/TMPRSS2 suggested: JCRB Cat# JCRB1819, RRID:CVCL_YQ49)
   Recombinant DNA
   Sentences	Resources
   The expression plasmid for the truncated S protein of SARS-CoV-2, pCAG-SARS-CoV-2 S (Wuhan), was kindly provided by Dr. Shuetsu Fukushi, National Institute of Infectious Diseases, Japan.	pCAG-SARS-CoV-2 suggested: None
   The expression plasmids for the truncated mutant S protein of SARS-CoV-2, pCAGG-pm3-SARS2-Shu-d19-B1.1.7 (B.1.1.7-derived variant), and pCAGG-pm3-SARS2-Shu-d19-B1.351 (B.1.351-derived variant), were constructed by PCR-based site-directed mutagenesis using cDNA as a template, which was obtained by chemical synthesis with optimisation for the humanised codon (Thermo Fisher Scientific, Waltham,	pCAGG-pm3-SARS2-Shu-d19-B1.1.7 suggested: None pCAGG-pm3-SARS2-Shu-d19-B1.351 suggested: None
   S cDNA of SARS-CoV-2 was cloned into the pCAGGS-pm3 expression vector.	pCAGGS-pm3 suggested: None
   Software and Algorithms
   Sentences	Resources
   Data were analysed using GraphPad Prism version 8.4.3 (GraphPad Software, San Diego, CA).	GraphPad Prism suggested: (GraphPad Prism, RRID:SCR_002798) GraphPad suggested: (GraphPad Prism, RRID:SCR_002798)

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   Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

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   Results from LimitationRecognizer: We detected the following sentences addressing limitations in the study:

   The limitations of the current study are as follows. The efficacy of SARS-CoV-2 vaccination among people whose immunity may be unstable, such as children, elderly people, and individuals with underlying diseases, is unknown. It is still unknown how long immunity is maintained after vaccination. Therefore, we plan to conduct a follow-up study for the participants. Lastly, it is impossible to exclude possible participants who had already acquired immunity because sera prior to vaccination were not assessed. In conclusion, the BNT162b2 vaccine produced a sufficient level of antibodies with a neutralising effect against WT SARS-CoV-2 and emerging variants. Antibody induction can be affected by age and sex but is still induced to a sufficient level.

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   Results from TrialIdentifier: No clinical trial numbers were referenced.

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   Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

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   Results from JetFighter: We did not find any issues relating to colormaps.

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   Results from rtransparent:

   • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
   • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
   • No protocol registration statement was detected.

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   Results from scite Reference Check: We found no unreliable references.

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3. 

   Version published to 10.1101/2021.05.26.21257884v1 on medRxiv

   May 30, 2021