Interplay of Antibody and Cytokine Production Reveals CXCL13 as a Potential Novel Biomarker of Lethal SARS-CoV-2 Infection
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Abstract
The SARS-CoV-2 pandemic is continuing to impact the global population, and knowledge of the immune response to COVID-19 is still developing. This study assesses the interplay of different parts of the immune system during COVID-19 disease.
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SciScore for 10.1101/2020.08.24.20180877: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Antibodies Sentences Resources Secondary antibody buffer (100μL of 1 % milk diluted in PBS-T containing 1:500 goat anti-human-IgG-HRP; Invitrogen Part #: 31410) was added immediately following the washing procedure. anti-human-IgG-HRPsuggested: NoneExperimental Models: Cell Lines Sentences Resources SARS-CoV-2 RBD protein was produced by transient transfection of HEK293T cells cultured in 300 cm2 flasks. HEK293Tsuggested: NoneSoftware and Algorithms Sentences Resources Serological results … SciScore for 10.1101/2020.08.24.20180877: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Antibodies Sentences Resources Secondary antibody buffer (100μL of 1 % milk diluted in PBS-T containing 1:500 goat anti-human-IgG-HRP; Invitrogen Part #: 31410) was added immediately following the washing procedure. anti-human-IgG-HRPsuggested: NoneExperimental Models: Cell Lines Sentences Resources SARS-CoV-2 RBD protein was produced by transient transfection of HEK293T cells cultured in 300 cm2 flasks. HEK293Tsuggested: NoneSoftware and Algorithms Sentences Resources Serological results from EUA approved antibody testing (Abbott Architect) performed in the WVUH clinical laboratory and ABO blood type were documented when available. Abbott Architectsuggested: (Abbott ARCHITECT i1000sr System, RRID:SCR_019328)Principal component and heatmap analysis: Serological data from patients tested for cytokine production and antibody production were pooled into Microsoft Excel and imported to ClustVis17. Microsoft Excelsuggested: (Microsoft Excel, RRID:SCR_016137)Pearson correlation coefficients and p-values were calculated in GraphPad Prism using the “Correlation” analysis. GraphPad Prismsuggested: (GraphPad Prism, RRID:SCR_002798)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
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