Human Basigin (CD147) Does Not Directly Interact with SARS-CoV-2 Spike Glycoprotein

  1. Robert J. Ragotte
  2. David Pulido
  3. Francesca R. Donnellan
  4. Michelle L. Hill
  5. Giacomo Gorini
  6. Hannah Davies
  7. Juliane Brun
  8. Kirsty McHugh
  9. Lloyd D. W. King
  10. Katherine Skinner
  11. Kazutoyo Miura
  12. Carole A. Long
  13. Nicole Zitzmann
  14. Simon J. Draper

Reviewed by

Read the full article See related articles

Listed in

Log in to save this article

Abstract

Reducing the mortality and morbidity associated with COVID-19 remains a global health priority. Vaccines have proven highly effective at preventing infection and hospitalization, but efforts must continue to improve treatment options for those who still become seriously ill.

Article activity feed

  1. Version published to 10.1128/msphere.00647-21
  2. ScreenIT

    SciScore for 10.1101/2021.02.22.432402: (What is this?)

    Please note, not all rigor criteria are appropriate for all manuscripts.

    Table 1: Rigor

    Institutional Review Board Statementnot detected.
    Randomizationnot detected.
    Blindingnot detected.
    Power Analysisnot detected.
    Sex as a biological variablenot detected.
    Cell Line Authenticationnot detected.

    Table 2: Resources

    Antibodies
    SentencesResources
    Human IgG1 CR3022 antibody (28) (GenBank: ABA54613.1 and ABA54614.1) was expressed from heavy and light chain AbVec expression vectors in Expi293™ cells (Thermo Fisher Scientific).
    Human IgG1 CR3022 antibody
    suggested: (Imported from the IEDB Cat# CR3022, RRID:AB_2848080)
    Human IgG1
    suggested: (Imported from the IEDB Cat# CR3022, RRID:AB_2848080)
    Experimental Models: Cell Lines
    SentencesResources
    This construct was expressed as a secreted protein by a stable Drosophila S2 cell line (30) and affinity purified using C-tag affinity resin (Thermo Fisher Scientific) followed by a size-exclusion chromatography polishing step in 20 mM Tris, 150 mM NaCl, pH 7.4.
    S2
    suggested: DGRC Cat# 150, RRID:CVCL_Z831)

    Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

    Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

    Results from TrialIdentifier: We found the following clinical trial numbers in your paper:

    IdentifierStatusTitle
    NCT04275245RecruitingClinical Study of Anti-CD147 Humanized Meplazumab for Inject…

    Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

    Results from JetFighter: We did not find any issues relating to colormaps.

    Results from rtransparent:
    • No conflict of interest statement was detected. If there are no conflicts, we encourage authors to explicit state so.
    • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
    • No protocol registration statement was detected.

    About SciScore

    SciScore is an automated tool that is designed to assist expert reviewers by finding and presenting formulaic information scattered throughout a paper in a standard, easy to digest format. SciScore checks for the presence and correctness of RRIDs (research resource identifiers), and for rigor criteria such as sex and investigator blinding. For details on the theoretical underpinning of rigor criteria and the tools shown here, including references cited, please follow this link.

  3. Version published to 10.1101/2021.02.22.432402v1 on bioRxiv