Aerosolized Hydrogen Peroxide Decontamination of N95 Respirators, with Fit-Testing and Viral Inactivation, Demonstrates Feasibility for Reuse during the COVID-19 Pandemic
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Abstract
The COVID-19 pandemic led to unprecedented pressure on health care and research facilities to provide personal protective equipment. The respiratory nature of the SARS-CoV2 pathogen makes respirator facepieces a critical protective measure to limit inhalation of this virus.
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SciScore for 10.1101/2020.04.17.20068577: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement IACUC: This facility is a purpose-built BSL3 enhanced facility, and all required approvals were obtained from the Institutional Biosafety Committee (IBC) for work involving viruses, as described below. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable Since the study was conducted during the COVID-19 pandemic, social distancing conditions limited the use of multiple test subjects, therefore, two respirator fit-test subjects were selected (one male, one female) for fit-testing of selected respirators. Cell Line Authentication not detected. Table 2: Resources
Experimental Models: Cell Lines Sentences Resources After … SciScore for 10.1101/2020.04.17.20068577: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement IACUC: This facility is a purpose-built BSL3 enhanced facility, and all required approvals were obtained from the Institutional Biosafety Committee (IBC) for work involving viruses, as described below. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable Since the study was conducted during the COVID-19 pandemic, social distancing conditions limited the use of multiple test subjects, therefore, two respirator fit-test subjects were selected (one male, one female) for fit-testing of selected respirators. Cell Line Authentication not detected. Table 2: Resources
Experimental Models: Cell Lines Sentences Resources After resuspension, viral titer was determined by tissue culture infectious dose 50 (TCID50) assay in 96-well plates, using Vero E6 cells. Vero E6suggested: RRID:CVCL_XD71)Software and Algorithms Sentences Resources Decontamination Facility: The decontamination process was carried out in the Eva J. Pell Laboratory for Advanced Biological Research at The Pennsylvania State University, University Park campus. Advanced Biologicalsuggested: NoneSpore-based Biological Indicators: Biological indicator (BI) spore discs (Steris Spordex®) inoculated with Geobacillus stearothermophilus (ATCC® 7953; mean spore count 2.4 x 105) were used to verify successful decontamination (34) Spore-based Biologicalsuggested: NoneThe PortaSens was also used to monitor Prep Room concentrations at the start of the aeration phase, and to verify concentrations were reduced to less than 1 ppm for safe re-entry to the room (without respiratory protection). PortaSenssuggested: NoneAnalysis was subcontracted and completed by laboratories participating in the American Industrial Hygiene Association (AIHA) Laboratory Accreditation Program. Laboratory Accreditation Programsuggested: NoneResults from OddPub: Thank you for sharing your data.
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
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