Antibody and Memory B-Cell Immunity in a Heterogeneously SARS-CoV-2-Infected and -Vaccinated Population

  1. Eva Bednarski
  2. Perla M. Del Rio Estrada
  3. Justin DaSilva
  4. Celia Boukadida
  5. Fengwen Zhang
  6. Yara A. Luna-Villalobos
  7. Ximena Rodríguez-Rangel
  8. Elvira Pitén-Isidro
  9. Edgar Luna-García
  10. Dafne Díaz Rivera
  11. Dulce M. López-Sánchez
  12. Daniela Tapia-Trejo
  13. Maribel Soto-Nava
  14. Myriam Astorga-Castañeda
  15. José O. Martínez-Moreno
  16. Guadalupe S. Urbina-Granados
  17. José A. Jiménez-Jacinto
  18. Francisco J. Serna Alvarado
  19. Yerania E. Enriquez-López
  20. Oliva López-Arellano
  21. Gustavo Reyes-Teran
  22. Paul D. Bieniasz
  23. Santiago Avila-Rios
  24. Theodora Hatziioannou

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Abstract

The majority of studies on SARS-CoV-2 vaccine-elicited immunity and immune evasion have focused on single vaccines corresponding to those distributed in high-income countries. However, in low- and middle-income countries, vaccine deployment has been far less uniform.

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  1. Version published to 10.1128/mbio.00840-22
  2. ScreenIT

    SciScore for 10.1101/2022.02.07.22270626: (What is this?)

    Please note, not all rigor criteria are appropriate for all manuscripts.

    Table 1: Rigor

    EthicsConsent: All donors provided written informed consent in compliance with protocols set forth and approved by the Comité de Ética en Investigación and the Comité de Investigación (Research Ethics Committee and the Research Committee) from INER Institutional Review Board (study no. B01-21).
    IRB: All donors provided written informed consent in compliance with protocols set forth and approved by the Comité de Ética en Investigación and the Comité de Investigación (Research Ethics Committee and the Research Committee) from INER Institutional Review Board (study no. B01-21).
    Sex as a biological variablenot detected.
    Randomizationnot detected.
    Blindingnot detected.
    Power Analysisnot detected.
    Cell Line Authenticationnot detected.

    Table 2: Resources

    Antibodies
    SentencesResources
    Additionally, SARS-CoV-2 infection was evaluated in all participants by detection of anti-N antibodies in plasma by ELISA (Elecsys Anti-SARS-CoV-2. Roche, Cat No 09203095190).
    anti-N
    suggested: None
    A purified human monoclonal antibody against SARS-CoV-2 Spike, C144, diluted 1:20 in 2% BSA/PBS and serially diluted, was used as a positive control.
    SARS-CoV-2
    suggested: None
    C144
    suggested: None
    50μl of reporter lysis buffer (Promega) was added to the beads and antibody-captured NanoLuc luciferase activity measured using the Nano-Glo Luciferase Assay System (Promega) with the Glomax Navigator (Promega).
    antibody-captured NanoLuc luciferase
    suggested: None
    Experimental Models: Cell Lines
    SentencesResources
    Briefly, 293Tcells were transfected with pNL4-3ΔEnv-nanoluc and pSARS-CoV-2-SΔ19.
    293Tcells
    suggested: None
    The S-6P-NanoLuc protein was expressed in 293-Expi cells, captured on Ni2+-magnetic beads and the purified protein eluted following incubation with 3CL protease.
    293-Expi
    suggested: None
    Recombinant DNA
    SentencesResources
    Briefly, 293Tcells were transfected with pNL4-3ΔEnv-nanoluc and pSARS-CoV-2-SΔ19.
    pNL4-3ΔEnv-nanoluc
    suggested: None
    pSARS-CoV-2-SΔ19
    suggested: None
    Software and Algorithms
    SentencesResources
    Non-parametric Spearmen’s correlation analysis (GraphPad Prism) was performed and p values calculated with a two-tailed analysis with 95% confidence interval.
    GraphPad
    suggested: (GraphPad Prism, RRID:SCR_002798)
    Stained PBMC were acquired in a BD FACS Fusion using FACS DIVA software (v8.0) and data were analyzed using FloJo v10.8.0 software.
    FloJo
    suggested: None

    Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

    Results from LimitationRecognizer: We detected the following sentences addressing limitations in the study:
    This information could also guide the use of vaccines that are not widely used in wealthier nations and broaden options when confronting inequity in vaccine distribution, production limitations as well as hesitancy issues.

    Results from TrialIdentifier: No clinical trial numbers were referenced.

    Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

    Results from JetFighter: We did not find any issues relating to colormaps.

    Results from rtransparent:
    • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
    • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
    • No protocol registration statement was detected.

    Results from scite Reference Check: We found no unreliable references.

    About SciScore

    SciScore is an automated tool that is designed to assist expert reviewers by finding and presenting formulaic information scattered throughout a paper in a standard, easy to digest format. SciScore checks for the presence and correctness of RRIDs (research resource identifiers), and for rigor criteria such as sex and investigator blinding. For details on the theoretical underpinning of rigor criteria and the tools shown here, including references cited, please follow this link.

  3. Version published to 10.1101/2022.02.07.22270626 on medRxiv