Convalescent-Phase Sera and Vaccine-Elicited Antibodies Largely Maintain Neutralizing Titer against Global SARS-CoV-2 Variant Spikes

  1. Takuya Tada
  2. Belinda M. Dcosta
  3. Marie I. Samanovic
  4. Ramin S. Herati
  5. Amber Cornelius
  6. Hao Zhou
  7. Ada Vaill
  8. Wes Kazmierski
  9. Mark J. Mulligan
  10. Nathaniel R. Landau

Reviewed by

Read the full article See related articles

Listed in

Log in to save this article

Abstract

The rapid evolution of SARS-CoV-2 variants has raised concerns with regard to their potential to escape from vaccine-elicited antibodies and anti-spike protein monoclonal antibodies. We report here on an analysis of sera from recovered patients and vaccinated individuals and on neutralization by Regeneron therapeutic monoclonal antibodies.

Article activity feed

  1. Version published to 10.1128/mbio.00696-21
  2. ScreenIT

    SciScore for 10.1101/2021.02.05.430003: (What is this?)

    Please note, not all rigor criteria are appropriate for all manuscripts.

    Table 1: Rigor

    Institutional Review Board StatementConsent: Human sera: Convalescent sera and sera from individuals vaccinated at NYULH with BNT162b2 on day 0, day 7 and day 28 (7 days following the second injection) were collected from individuals through the NYU Vaccine Center with written consent under I.R.B. approval (IRB 20-00595 and IRB 18-02037) and were deidentified.
    Randomizationnot detected.
    Blindingnot detected.
    Power Analysisnot detected.
    Sex as a biological variablenot detected.
    Cell Line Authenticationnot detected.

    Table 2: Resources

    Antibodies
    SentencesResources
    anti-p24 mAb (AG3.0), anti-His mAb (Invitrogen) and anti-GAPDH mAb (Life Technologies) followed by goat anti-mouse HRP-conjugated second antibody (Sigma).
    anti-His
    suggested: None
    anti-GAPDH
    suggested: None
    anti-mouse
    suggested: None
    The beads were then washed with PBS, resuspended in reducing Laemmle loading buffer, heated to 90°C and separated by SDS-PAGE and analyzed on an immunoblot probed with anti-p24 antibody (AG3.0) followed by goat anti-mouse HRP-conjugated secondly antibody.
    anti-p24
    suggested: None
    anti-mouse HRP-conjugated secondly antibody.
    suggested: None
    Experimental Models: Cell Lines
    SentencesResources
    Cells: 293T cells were cultured in Dulbecco’s modified Eagle medium (DMEM) supplemented with 10% fetal bovine serum (FBS) and penicillin/streptomycin (P/S) at 37°C in 5% CO2.
    293T
    suggested: None
    ACE2.293T cells are clonal cell-lines established by stable co-transfection with pLenti.ACE2-HA followed by selection in 1 μg/ml puromycin, as previously described 23,25.
    ACE2.293T
    suggested: None
    Software and Algorithms
    SentencesResources
    Quantification and Statistical Analysis: All experiments were performed in technical duplicates or triplicates and data were analyzed using GraphPad Prism (Version 8).
    GraphPad Prism
    suggested: (GraphPad Prism, RRID:SCR_002798)

    Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

    Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

    Results from TrialIdentifier: No clinical trial numbers were referenced.

    Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

    Results from JetFighter: We did not find any issues relating to colormaps.

    Results from rtransparent:
    • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
    • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
    • No protocol registration statement was detected.

    About SciScore

    SciScore is an automated tool that is designed to assist expert reviewers by finding and presenting formulaic information scattered throughout a paper in a standard, easy to digest format. SciScore checks for the presence and correctness of RRIDs (research resource identifiers), and for rigor criteria such as sex and investigator blinding. For details on the theoretical underpinning of rigor criteria and the tools shown here, including references cited, please follow this link.

  3. Version published to 10.1101/2021.02.05.430003v1 on bioRxiv