COVID-19 Severity Is Associated with Differential Antibody Fc-Mediated Innate Immune Functions

Article activity feed

1. Version published to 10.1128/mbio.00281-21

   Apr 27, 2021
2. 

   ScreenIT

   Jan 12, 2021

   SciScore for 10.1101/2021.01.11.426209: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Institutional Review Board Statement	IRB: Ethics: All research protocols of the study were approved by the institutional review boards (IRB) at Rush University and The Wistar Institute.
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Sex as a biological variable	The cohort was also chosen to be 45–60% female per group.
   Cell Line Authentication	not detected.

   Table 2: Resources

   Antibodies
   Sentences	Resources
   ADCD measurements: Purified IgG antibodies were analyzed for their ability to fix complement on target cells.	Purified IgG suggested: None
   Experimental Models: Cell Lines
   Sentences	Resources
   4 × 106 (ACE2)-CHO cells were pulsed with 20 μg biotinylated SARS-CoV-2 S1 or RBD proteins …

   SciScore for 10.1101/2021.01.11.426209: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Institutional Review Board Statement	IRB: Ethics: All research protocols of the study were approved by the institutional review boards (IRB) at Rush University and The Wistar Institute.
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Sex as a biological variable	The cohort was also chosen to be 45–60% female per group.
   Cell Line Authentication	not detected.

   Table 2: Resources

   Antibodies
   Sentences	Resources
   ADCD measurements: Purified IgG antibodies were analyzed for their ability to fix complement on target cells.	Purified IgG suggested: None
   Experimental Models: Cell Lines
   Sentences	Resources
   4 × 106 (ACE2)-CHO cells were pulsed with 20 μg biotinylated SARS-CoV-2 S1 or RBD proteins (Acro Biosystems) for 30 min at 37°C.	ACE2)-CHO suggested: None
   A 200 μl suspension of THP-1 cells at 2.5 × 105 cells/ml was added to each well, for a total of 5 × 104 THP-1 cells per well.	THP-1 suggested: None
   Software and Algorithms
   Sentences	Resources
   Cells were analyzed by flow cytometry, and data collected were analyzed in FlowJo software.	FlowJo suggested: (FlowJo, RRID:SCR_008520)
   Statistical analyses were performed in Prism 7.0 (GraphPad).	Prism suggested: (PRISM, RRID:SCR_005375) GraphPad suggested: (GraphPad Prism, RRID:SCR_002798)

   ---

   Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

   ---

   Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

   ---

   Results from TrialIdentifier: No clinical trial numbers were referenced.

   ---

   Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

   ---

   Results from JetFighter: We did not find any issues relating to colormaps.

   ---

   Results from rtransparent:

   • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
   • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
   • No protocol registration statement was detected.

   ---

   Read the original source
3. 

   Version published to 10.1101/2021.01.11.426209 on bioRxiv

   Jan 11, 2021