Development of Spike Receptor-Binding Domain Nanoparticles as a Vaccine Candidate against SARS-CoV-2 Infection in Ferrets

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1. Version published to 10.1128/mbio.00230-21

   Apr 27, 2021
2. 

   ScreenIT

   Mar 1, 2021

   SciScore for 10.1101/2021.01.28.428743: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Institutional Review Board Statement	IACUC: All animal cares were performed strictly following the animal care guideline and experiment protocols approved by the Institutional Animal Care and Use Committee (IACUC) in Chungbuk National University
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Sex as a biological variable	Animal Care: Male and female ferrets of 16-20 months old and tested seronegative for Influenza A, MERS-CoV, and SARS-CoV were purchased from ID Bio Corporation (Cheongju, Korea).
   Cell Line Authentication	not detected.

   Table 2: Resources

   Experimental Models: Cell Lines
   Sentences	Resources
   Expression and purification of nanoparticles: HEK293T cells were directly …

   SciScore for 10.1101/2021.01.28.428743: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Institutional Review Board Statement	IACUC: All animal cares were performed strictly following the animal care guideline and experiment protocols approved by the Institutional Animal Care and Use Committee (IACUC) in Chungbuk National University
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Sex as a biological variable	Animal Care: Male and female ferrets of 16-20 months old and tested seronegative for Influenza A, MERS-CoV, and SARS-CoV were purchased from ID Bio Corporation (Cheongju, Korea).
   Cell Line Authentication	not detected.

   Table 2: Resources

   Experimental Models: Cell Lines
   Sentences	Resources
   Expression and purification of nanoparticles: HEK293T cells were directly purchased from American Type Culture Collection (ATCC) and maintained in DMEM medium (Gibco) supplemented with 10% FBS (Gibco) and 1% penicillin/streptomycin (Gibco).	HEK293T suggested: None
   Infectious viruses from the nasal washes and lung tissues were quantified by inoculation onto Vero cells.	Vero suggested: CLS Cat# 605372/p622_VERO, RRID:CVCL_0059)
   Software and Algorithms
   Sentences	Resources
   Computer-assisted three-dimensional model of nanoparticles: Previously solved structures of H. pylori ferritin nanoparticle (PDB: 3EGM) and SARS-CoV-2 RBD (PDB: 7JMP) were processed with PyMol (Schrodinger) and Autodesk Meshmixer (Autodesk).	PyMol suggested: (PyMOL, RRID:SCR_000305)
   Statistical Analysis: All figure asterisks indicate statistical significance compared with adjuvant-only group as evaluated by the two-way ANOVA Dunnett’s multiple comparisons tests (* indicates p<0.05, ** indicates p<0.01, *** indicates p<0.001 and **** indicates p<0.0001) and were drawn using GraphPad Prism 8 (GraphPad).	GraphPad Prism suggested: (GraphPad Prism, RRID:SCR_002798) GraphPad suggested: (GraphPad Prism, RRID:SCR_002798)

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   Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

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   Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

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   Results from TrialIdentifier: No clinical trial numbers were referenced.

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   Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

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   Results from JetFighter: We did not find any issues relating to colormaps.

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   Results from rtransparent:

   • No conflict of interest statement was detected. If there are no conflicts, we encourage authors to explicit state so.
   • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
   • No protocol registration statement was detected.

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3. 

   Version published to 10.1101/2021.01.28.428743 on bioRxiv

   Jan 29, 2021