Transmission and Protection against Reinfection in the Ferret Model with the SARS-CoV-2 USA-WA1/2020 Reference Isolate

Article activity feed

1. Version published to 10.1128/jvi.02232-20

   Jun 10, 2021
2. 

   ScreenIT

   Nov 22, 2020

   SciScore for 10.1101/2020.11.20.392381: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Institutional Review Board Statement	IACUC: All animal studies were approved by the PSU Animal Care and Use Committee under protocol no. 202001440.
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Sex as a biological variable	Equal number of male and female ferrets, 24-30 weeks old (Triple F Farms, Sayre, PA) were used for all studies.
   Cell Line Authentication	not detected.

   Table 2: Resources

   Antibodies
   Sentences	Resources
   S-protein RBD ELISA: To quantify anti-S protein RBD antibodies from ferret serum, 96-well ELISA plates (Nunc) were coated overnight with 2.0 ug/mL (100ng/well) of SARS-CoV-2 RBD S Protein (Sino Biological) in sodium bicarbonate buffer.	anti-S protein RBD suggested: …

   SciScore for 10.1101/2020.11.20.392381: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Institutional Review Board Statement	IACUC: All animal studies were approved by the PSU Animal Care and Use Committee under protocol no. 202001440.
   Randomization	not detected.
   Blinding	not detected.
   Power Analysis	not detected.
   Sex as a biological variable	Equal number of male and female ferrets, 24-30 weeks old (Triple F Farms, Sayre, PA) were used for all studies.
   Cell Line Authentication	not detected.

   Table 2: Resources

   Antibodies
   Sentences	Resources
   S-protein RBD ELISA: To quantify anti-S protein RBD antibodies from ferret serum, 96-well ELISA plates (Nunc) were coated overnight with 2.0 ug/mL (100ng/well) of SARS-CoV-2 RBD S Protein (Sino Biological) in sodium bicarbonate buffer.	anti-S protein RBD suggested: (Imported from the IEDB Cat# RBD-25E4, RRID:AB_2848097)
   Plates were washed again and incubated for 2 hours at room temperature with HRP conjugated anti-ferret goat antibody (Rockland) diluted 1:10,000 in PBST and 1% skim milk powder.	anti-ferret goat antibody (Rockland) suggested: None
   Experimental Models: Cell Lines
   Sentences	Resources
   To assess peak titers in the nasal wash samples, 10-fold serial dilutions of virus were titrated in 96-well plates of Vero E6 cells.	Vero E6 suggested: RRID:CVCL_XD71)
   Software and Algorithms
   Sentences	Resources
   Biocontainment and Animal Care and Use: All experiments with SARS-CoV-2 were conducted in the Eva Pell Laboratory for Advanced Biological Research which is the Pennsylvania State University’s biosafety level 3 enhanced laboratory.	Advanced Biological suggested: None
   Statistical analysis was performed with Prism GraphPad (v8.4.3) and p<0.05 considered statistically significant.	GraphPad suggested: (GraphPad Prism, RRID:SCR_002798)

   ---

   Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

   ---

   Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

   ---

   Results from TrialIdentifier: No clinical trial numbers were referenced.

   ---

   Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

   ---

   Results from JetFighter: We did not find any issues relating to colormaps.

   ---

   Results from rtransparent:

   • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
   • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
   • No protocol registration statement was detected.

   ---

   Read the original source
3. 

   Version published to 10.1101/2020.11.20.392381v1 on bioRxiv

   Nov 22, 2020