Saliva Is a Promising Alternative Specimen for the Detection of SARS-CoV-2 in Children and Adults
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Abstract
Testing efforts for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) have been burdened by the scarcity of testing materials and personal protective equipment for health care workers. The simple and painless process of saliva collection allows for widespread testing, but enthusiasm is hampered by variable performance compared to that of nasopharyngeal swab (NPS) samples.
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SciScore for 10.1101/2020.10.25.20219055: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement IRB: Study design conducted at Children’s Hospital Los Angeles was approved by the Institutional Review Board under IRB #CHLA-20-00124 and CHLA-18-00098. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Table 2: Resources
Software and Algorithms Sentences Resources Total nucleic acid was extracted from 250 µL samples using the Thermo Fisher KingFisher Flex specimen processing system with the Applied Biosystems MagMAX Viral/Pathogen Nucleic Acid Isolation Kit (Thermo Fisher, Waltham, MA) and eluted to 50 µL of total nucleic acid. Thermo Fisher KingFishersuggested: NoneResults from OddPub: We did not …
SciScore for 10.1101/2020.10.25.20219055: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement IRB: Study design conducted at Children’s Hospital Los Angeles was approved by the Institutional Review Board under IRB #CHLA-20-00124 and CHLA-18-00098. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Table 2: Resources
Software and Algorithms Sentences Resources Total nucleic acid was extracted from 250 µL samples using the Thermo Fisher KingFisher Flex specimen processing system with the Applied Biosystems MagMAX Viral/Pathogen Nucleic Acid Isolation Kit (Thermo Fisher, Waltham, MA) and eluted to 50 µL of total nucleic acid. Thermo Fisher KingFishersuggested: NoneResults from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: We detected the following sentences addressing limitations in the study:Limitations of this study include the small sample size of both children, particularly younger children, and adults from a single medical institution. Second, this study consisted of only outpatients, patients admitted to the emergency department, and family members who volunteered to enroll in the study which can bias our findings regarding the role of COVID-19 exposure to specimen performance. Since viral load may or may not be correlated with clinical manifestations, further studies should be conducted in inpatient or ICU settings as the spectrum of disease ranges from asymptomatic to severely ill patients (21-23). Finally, despite a standardized protocol utilized during the collection of the saliva samples, it can be challenging for children to properly salivate into a collection device. The volume of saliva obtained may also vary among patients due to excessive bubbles and other factors despite the same amount of saliva being processed for testing.
Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
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