Quantitative SARS-CoV-2 Viral-Load Curves in Paired Saliva Samples and Nasal Swabs Inform Appropriate Respiratory Sampling Site and Analytical Test Sensitivity Required for Earliest Viral Detection
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Abstract
Early detection of SARS-CoV-2 infection is critical to reduce asymptomatic and presymptomatic transmission, curb the spread of variants, and maximize treatment efficacy. Low-analytical-sensitivity nasal-swab testing is commonly used for surveillance and symptomatic testing, but the ability of these tests to detect the earliest stages of infection has not been established.
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SciScore for 10.1101/2021.04.02.21254771: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement IRB: 12 Both studies were reviewed and approved by the Institutional Review Board of the California Institute of Technology, protocol #20-1026.
Consent: All participants provided either written informed consent or (for minors ages 6-17) assent accompanied by parental permission, prior to enrollment.Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Table 2: Resources
Software and Algorithms Sentences Resources All participant data were collected and managed using REDCap (Research Electronic Data Capture) on a server hosted at the California Institute of Technology. REDCapsuggested: (REDCap, RRID:SCR_0034…SciScore for 10.1101/2021.04.02.21254771: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement IRB: 12 Both studies were reviewed and approved by the Institutional Review Board of the California Institute of Technology, protocol #20-1026.
Consent: All participants provided either written informed consent or (for minors ages 6-17) assent accompanied by parental permission, prior to enrollment.Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Table 2: Resources
Software and Algorithms Sentences Resources All participant data were collected and managed using REDCap (Research Electronic Data Capture) on a server hosted at the California Institute of Technology. REDCapsuggested: (REDCap, RRID:SCR_003445)Reactions were run in duplicate on a CFX96 Real Time Instrument (Bio-Rad Laboratories, Hercules, CA, USA). Bio-Rad Laboratoriessuggested: (Bio-Rad Laboratories, RRID:SCR_008426)Samples were analyzed with QuantaSoft analysis Pro 1.0.595 software following Bio-Rad’s RUO SARS-CoV-2 guidelines.43 QuantaSoft analysis Prosuggested: NoneResults from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
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