SARS-CoV-2 Assays To Detect Functional Antibody Responses That Block ACE2 Recognition in Vaccinated Animals and Infected Patients

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1. Version published to 10.1128/jcm.01533-20

   Oct 21, 2020
2. 

   ScreenIT

   Jun 21, 2020

   SciScore for 10.1101/2020.06.17.158527: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Institutional Review Board Statement	not detected.
   Randomization	not detected.
   Blinding	Samples were not blinded before performing each experiment.
   Power Analysis	not detected.
   Sex as a biological variable	not detected.
   Cell Line Authentication	not detected.

   Table 2: Resources

   Experimental Models: Cell Lines
   Sentences	Resources
   SARS-CoV-2 Pseudovirus assay: The SARS-CoV-2 pseudovirus was produced by co-transfection of HEK293T cells with 1:1 ratio of DNA plasmid encoding SARS-CoV-2 S protein (GenScript) and backbone plasmid pNL4-3.	HEK293T suggested: None
   For neutralization assay, serially diluted samples were incubated with pseudovirus at room temperature for 90 minutes and added to 10,000 ACE2-293T cells in 200uL …

   SciScore for 10.1101/2020.06.17.158527: (What is this?)

   Please note, not all rigor criteria are appropriate for all manuscripts.

   Table 1: Rigor

   Institutional Review Board Statement	not detected.
   Randomization	not detected.
   Blinding	Samples were not blinded before performing each experiment.
   Power Analysis	not detected.
   Sex as a biological variable	not detected.
   Cell Line Authentication	not detected.

   Table 2: Resources

   Experimental Models: Cell Lines
   Sentences	Resources
   SARS-CoV-2 Pseudovirus assay: The SARS-CoV-2 pseudovirus was produced by co-transfection of HEK293T cells with 1:1 ratio of DNA plasmid encoding SARS-CoV-2 S protein (GenScript) and backbone plasmid pNL4-3.	HEK293T suggested: None
   For neutralization assay, serially diluted samples were incubated with pseudovirus at room temperature for 90 minutes and added to 10,000 ACE2-293T cells in 200uL TPCK media (DMEM supplemented with 1%BSA, 25mM HEPES, 1ug/ml of TPCK and 1X Penicillin-Streptomycin) in 96-well tissue culture plates, and incubated at 37C and 5% CO2 for 72 hours.	ACE2-293T suggested: None
   Software and Algorithms
   Sentences	Resources
   The data collected was analyzed using the protein conjugated analysis from ASTRA software (Wyatt technology).	ASTRA suggested: (ASTRA, RRID:SCR_016255)
   The regeneration solution was made using 3-parts 8M guanidine hydrochloride and 1-part 1M sodium hydroxide. Statistics: Statistical analyses were performed using GraphPad Prism 8 software.	GraphPad Prism suggested: (GraphPad Prism, RRID:SCR_002798)

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   Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

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   Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

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   Results from TrialIdentifier: No clinical trial numbers were referenced.

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   Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

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   Results from JetFighter: We did not find any issues relating to colormaps.

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   Results from rtransparent:

   • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
   • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
   • No protocol registration statement was detected.

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3. 

   Version published to 10.1101/2020.06.17.158527v1 on bioRxiv

   Jun 20, 2020