A SARS-CoV-2 Label-Free Surrogate Virus Neutralization Test and a Longitudinal Study of Antibody Characteristics in COVID-19 Patients
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Abstract
Methods designed to measure severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) humoral response include virus neutralization tests to determine antibody neutralization activity. For ease of use and universal applicability, surrogate virus neutralization tests (sVNTs) based on antibody-mediated blockage of molecular interactions have been proposed. A surrogate virus neutralization test was established on a label-free immunoassay platform (LF-sVNT).
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SciScore for 10.1101/2021.01.19.21250137: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement IRB: The study protocol was approved by the Institutional Review Board of the University of California San Francisco.
Consent: The committee deemed that written consent was not required for use of remnant specimens.Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable The patients were 63% male and 75% Hispanic, with a median age of 51 years. Cell Line Authentication not detected. Table 2: Resources
Antibodies Sentences Resources A human monoclonal anti-RBD IgG1 antibody was obtained from Absolute Antibody (Oxford, UK), and a goat anti-human IgG antibody (anti-IgG) from Jackson Immunoresearch (West Grove, PA). A human …SciScore for 10.1101/2021.01.19.21250137: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement IRB: The study protocol was approved by the Institutional Review Board of the University of California San Francisco.
Consent: The committee deemed that written consent was not required for use of remnant specimens.Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable The patients were 63% male and 75% Hispanic, with a median age of 51 years. Cell Line Authentication not detected. Table 2: Resources
Antibodies Sentences Resources A human monoclonal anti-RBD IgG1 antibody was obtained from Absolute Antibody (Oxford, UK), and a goat anti-human IgG antibody (anti-IgG) from Jackson Immunoresearch (West Grove, PA). A human monoclonal anti-RBD IgG1 antibodysuggested: Noneanti-RBD IgG1suggested: Noneanti-human IgGsuggested: Noneanti-IgGsuggested: NoneThe LF-sVNT analyzes the binding ability of RBD to ACE2 after neutralizing RBD with antibodies in serum. ACE2suggested: NoneExperimental Models: Cell Lines Sentences Resources The mixture was then added to a Vero cell suspension and incubated for 45 min. Verosuggested: NoneSoftware and Algorithms Sentences Resources The Pylon 3D fluorescence immunoassay analyzer was manufactured by ET Healthcare (Palo Alto, CA) ET Healthcaresuggested: NoneResults from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
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