Performance of COVIDSeq and Swift Normalase Amplicon SARS-CoV-2 Panels for SARS-CoV-2 Genome Sequencing: Practical Guide and Combining FASTQ Strategy

  1. Hosoon Choi
  2. Munok Hwang
  3. Dhammika H. Navarathna
  4. Jing Xu
  5. Janell Lukey
  6. Chetan Jinadatha

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  1. Version published to 10.1128/jcm.00025-22
  2. ScreenIT

    SciScore for 10.1101/2021.12.30.21268553: (What is this?)

    Please note, not all rigor criteria are appropriate for all manuscripts.

    Table 1: Rigor

    Ethicsnot detected.
    Sex as a biological variablenot detected.
    Randomizationnot detected.
    Blindingnot detected.
    Power Analysisnot detected.

    Table 2: Resources

    Software and Algorithms
    SentencesResources
    and Swift normalase amplicon SARS-CoV-2 panels (SNAP) library prep kit (Swift biosciences, Ann Arbor, MI, USA, catalog number SN-5X296) according to the manufacture’s protocol.
    SNAP
    suggested: (SNAP, RRID:SCR_007936)
    The cDNAs synthesized with COVIDseq Test kit were used as starting materials for Swift library prep.
    Swift
    suggested: (Swift, RRID:SCR_013018)
    For detailed sequence analysis, NCBI BLAST (https://blast.ncbi.nlm.nih.gov/Blast.cgi) was used.
    BLAST
    suggested: (BLASTX, RRID:SCR_001653)
    https://blast.ncbi.nlm.nih.gov/Blast.cgi
    suggested: (TBLASTX, RRID:SCR_011823)

    Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

    Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

    Results from TrialIdentifier: No clinical trial numbers were referenced.

    Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

    Results from JetFighter: We did not find any issues relating to colormaps.

    Results from rtransparent:
    • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
    • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
    • No protocol registration statement was detected.

    Results from scite Reference Check: We found no unreliable references.

    About SciScore

    SciScore is an automated tool that is designed to assist expert reviewers by finding and presenting formulaic information scattered throughout a paper in a standard, easy to digest format. SciScore checks for the presence and correctness of RRIDs (research resource identifiers), and for rigor criteria such as sex and investigator blinding. For details on the theoretical underpinning of rigor criteria and the tools shown here, including references cited, please follow this link.

  3. Version published to 10.1101/2021.12.30.21268553v1 on medRxiv