Single-cell RNA sequencing reveals SARS-CoV-2 infection dynamics in lungs of African green monkeys
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Abstract
Single-cell RNA sequencing of lungs of SARS-CoV-2–infected African green monkeys reveals virus replication and host response dynamics in vivo.
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SciScore for 10.1101/2020.08.20.258087: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement IACUC: Ethics and biosafety statement: All animal experiments were approved by the Institutional Animal Care and Use Committee of Rocky Mountain Laboratories, NIH and carried out by certified staff in an Association for Assessment and Accreditation of Laboratory Animal Care (AAALAC) International accredited facility, according to the institution’s guidelines for animal use, following the guidelines and basic principles in the NIH Guide for the Care and Use of Laboratory Animals, the Animal Welfare Act, United States Department of Agriculture and the United States Public Health Service Policy on Humane Care and Use of Laboratory Animals. Randomization not detected. B… SciScore for 10.1101/2020.08.20.258087: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement IACUC: Ethics and biosafety statement: All animal experiments were approved by the Institutional Animal Care and Use Committee of Rocky Mountain Laboratories, NIH and carried out by certified staff in an Association for Assessment and Accreditation of Laboratory Animal Care (AAALAC) International accredited facility, according to the institution’s guidelines for animal use, following the guidelines and basic principles in the NIH Guide for the Care and Use of Laboratory Animals, the Animal Welfare Act, United States Department of Agriculture and the United States Public Health Service Policy on Humane Care and Use of Laboratory Animals. Randomization not detected. Blinding Histopathological analysis of tissue slides was performed by a board-certified veterinary pathologist blinded to the group assignment of the animals. Power Analysis not detected. Sex as a biological variable Study design: To evaluate the pathogenesis of SARS-CoV-2 in African green monkeys, eight adult African green monkeys (4 males, and 4 females, body weight 3.5-6kg) were inoculated via a combination of intranasal (0.5 ml per nostril), intratracheal (4 ml), oral (1 ml) and ocular (0.25 ml per eye) of a 4⨯105 TCID50/ml (3⨯108 genome copies/ml) virus dilution in sterile DMEM. Cell Line Authentication not detected. Table 2: Resources
Antibodies Sentences Resources Immunohistochemistry was performed using a custom-made rabbit antiserum against SARS-CoV-2 N at a 1:1000 dilution, using a CD68 clone KP1 mouse monoclonal antibody (Dako/Agilent cat #M0814) at a 1:100 dilution to identify macrophages, and using Cytokeratin clone AE1/AE3 mouse monoclonal antibody (Dako/Agilent cat #M3515) at a 1:100 dilution to identify epithelial cells. CD68suggested: NoneCytokeratinsuggested: NoneExperimental Models: Cell Lines Sentences Resources Absence of infectious virus after gamma-irradiation was confirmed in Vero E6 cells. Vero E6suggested: RRID:CVCL_XD71)Software and Algorithms Sentences Resources Graphs were generated either in ggplot2 (Wickham, 2016) or Prism V8. ggplot2suggested: (ggplot2, RRID:SCR_014601)Results from OddPub: Thank you for sharing your data.
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
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