Antibody signature induced by SARS-CoV-2 spike protein immunogens in rabbits

  1. Supriya Ravichandran
  2. Elizabeth M. Coyle
  3. Laura Klenow
  4. Juanjie Tang
  5. Gabrielle Grubbs
  6. Shufeng Liu
  7. Tony Wang
  8. Hana Golding
  9. Surender Khurana

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Abstract

Understanding antibody epitope diversity and affinity generated by SARS-CoV-2 spike antigens in rabbits can aid COVID-19 vaccine development.

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  1. Version published to 10.1126/scitranslmed.abc3539
  2. ScreenIT

    SciScore for 10.1101/2020.05.12.091918: (What is this?)

    Please note, not all rigor criteria are appropriate for all manuscripts.

    Table 1: Rigor

    Institutional Review Board Statementnot detected.
    Randomizationnot detected.
    BlindingAll SPR experiments were performed twice and the researchers performing the assay were blinded to sample identity.
    Power Analysisnot detected.
    Sex as a biological variablenot detected.
    Cell Line Authenticationnot detected.

    Table 2: Resources

    Antibodies
    SentencesResources
    After three washes with PBS/0.05% Tween 20, bound antibodies were detected with a donkey anti-rabbit IgG Fc-specific HRP-conjugated antibody (Jackson Immuno Research) After 1hr, plates were washed as before and OPD was added for 10min.
    anti-rabbit IgG
    suggested: None
    Antibody isotype analysis for the SARS-CoV-2 spike protein bound antibodies in the polyclonal serum was performed using SPR.
    SPR
    suggested: None
    Experimental Models: Cell Lines
    SentencesResources
    Recombinant purified proteins used in the study were either produced in HEK 293 mammalian cells (S1 and RBD) or insect cells (S1+S2 ectodomain and S2 domain).
    HEK 293
    suggested: CLS Cat# 300192/p777_HEK293, RRID:CVCL_0045)
    Pseudovirions were produced by co-transfection Lenti‐X 293T cells with pMLV-gag-pol, pFBluc, and pcDNA 3.1 SARS-CoV-2 S using Lipofectamine 3000.
    293T
    suggested: None
    For neutralization assay, 50 µL of SARS-CoV-2 S pseudovirions were pre-incubated with an equal volume of medium containing serum at varying dilutions at room temperature for 1 h, then virus-antibody mixtures were added to Vero E6 cells in a 96-well plate.
    Vero E6
    suggested: None

    Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).

    Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

    Results from TrialIdentifier: No clinical trial numbers were referenced.

    Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

    Results from JetFighter: We did not find any issues relating to colormaps.

    Results from rtransparent:
    • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
    • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
    • No protocol registration statement was detected.

    About SciScore

    SciScore is an automated tool that is designed to assist expert reviewers by finding and presenting formulaic information scattered throughout a paper in a standard, easy to digest format. SciScore checks for the presence and correctness of RRIDs (research resource identifiers), and for rigor criteria such as sex and investigator blinding. For details on the theoretical underpinning of rigor criteria and the tools shown here, including references cited, please follow this link.

  3. Version published to 10.1101/2020.05.12.091918v1 on bioRxiv