Antibody signature induced by SARS-CoV-2 spike protein immunogens in rabbits
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Abstract
Understanding antibody epitope diversity and affinity generated by SARS-CoV-2 spike antigens in rabbits can aid COVID-19 vaccine development.
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SciScore for 10.1101/2020.05.12.091918: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding All SPR experiments were performed twice and the researchers performing the assay were blinded to sample identity. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Antibodies Sentences Resources After three washes with PBS/0.05% Tween 20, bound antibodies were detected with a donkey anti-rabbit IgG Fc-specific HRP-conjugated antibody (Jackson Immuno Research) After 1hr, plates were washed as before and OPD was added for 10min. anti-rabbit IgGsuggested: NoneAntibody isotype analysis for the SARS-CoV-2 spike protein bound antibodies in the polyclonal … SciScore for 10.1101/2020.05.12.091918: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding All SPR experiments were performed twice and the researchers performing the assay were blinded to sample identity. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Antibodies Sentences Resources After three washes with PBS/0.05% Tween 20, bound antibodies were detected with a donkey anti-rabbit IgG Fc-specific HRP-conjugated antibody (Jackson Immuno Research) After 1hr, plates were washed as before and OPD was added for 10min. anti-rabbit IgGsuggested: NoneAntibody isotype analysis for the SARS-CoV-2 spike protein bound antibodies in the polyclonal serum was performed using SPR. SPRsuggested: NoneExperimental Models: Cell Lines Sentences Resources Recombinant purified proteins used in the study were either produced in HEK 293 mammalian cells (S1 and RBD) or insect cells (S1+S2 ectodomain and S2 domain). HEK 293suggested: CLS Cat# 300192/p777_HEK293, RRID:CVCL_0045)Pseudovirions were produced by co-transfection Lenti‐X 293T cells with pMLV-gag-pol, pFBluc, and pcDNA 3.1 SARS-CoV-2 S using Lipofectamine 3000. 293Tsuggested: NoneFor neutralization assay, 50 µL of SARS-CoV-2 S pseudovirions were pre-incubated with an equal volume of medium containing serum at varying dilutions at room temperature for 1 h, then virus-antibody mixtures were added to Vero E6 cells in a 96-well plate. Vero E6suggested: NoneResults from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
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