BNT162b2 vaccination induces durable SARS-CoV-2–specific T cells with a stem cell memory phenotype

  1. Gisella Guerrera
  2. Mario Picozza
  3. Silvia D’Orso
  4. Roberta Placido
  5. Marta Pirronello
  6. Alice Verdiani
  7. Andrea Termine
  8. Carlo Fabrizio
  9. Flavia Giannessi
  10. Manolo Sambucci
  11. Maria Pia Balice
  12. Carlo Caltagirone
  13. Antonino Salvia
  14. Angelo Rossini
  15. Luca Battistini
  16. Giovanna Borsellino

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Abstract

SARS-CoV-2 mRNA vaccination induces stem cell memory T cells specific for the virus that persist up to 6 months.

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  1. Version published to 10.1126/sciimmunol.abl5344
  2. ScreenIT

    SciScore for 10.1101/2021.09.27.462006: (What is this?)

    Please note, not all rigor criteria are appropriate for all manuscripts.

    Table 1: Rigor

    EthicsConsent: All donors signed informed consent forms approved by the Ethical Committee of the Santa Lucia Foundation.
    IRB: All donors signed informed consent forms approved by the Ethical Committee of the Santa Lucia Foundation.
    Sex as a biological variableTo avoid gender and age biases, an equal number of female and male volunteers, as well as an equal number of subjects 22-45 and 45-66 years old, was included.
    Randomizationnot detected.
    Blindingnot detected.
    Power Analysisnot detected.

    Table 2: Resources

    Antibodies
    SentencesResources
    Evaluation of anti SARS-CoV-2 Antibodies: The measurement of anti SARS-CoV-2 neutralizing Abs was performed by electrochemiluminescence sandwich immunoassay (ECLIA) through Roche Elecsys Anti-SARS-CoV-2 S (Roche diagnostics, Switzerland).
    Antibodies
    suggested: None
    anti SARS-CoV-2
    suggested: None
    Software and Algorithms
    SentencesResources
    Flow cytometry staining and acquisition: Post-culture cells were pelleted in V-bottom 96-well plates and resuspended in 30 μl of antibodies at pre-optimized concentrations and diluted in Brilliant Stain Buffer (BD Biosciences), then incubated for 15’ at RT.
    BD Biosciences
    suggested: (BD Biosciences, RRID:SCR_013311)
    Data was analyzed with FlowJo v.
    FlowJo
    suggested: (FlowJo, RRID:SCR_008520)
    Statistical analysis: Statistical analysis was performed with GraphPad Prism 9.
    GraphPad Prism
    suggested: (GraphPad Prism, RRID:SCR_002798)

    Results from OddPub: Thank you for sharing your data.

    Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.

    Results from TrialIdentifier: No clinical trial numbers were referenced.

    Results from Barzooka: We did not find any issues relating to the usage of bar graphs.

    Results from JetFighter: We did not find any issues relating to colormaps.

    Results from rtransparent:
    • Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
    • Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
    • No protocol registration statement was detected.

    Results from scite Reference Check: We found no unreliable references.

    About SciScore

    SciScore is an automated tool that is designed to assist expert reviewers by finding and presenting formulaic information scattered throughout a paper in a standard, easy to digest format. SciScore checks for the presence and correctness of RRIDs (research resource identifiers), and for rigor criteria such as sex and investigator blinding. For details on the theoretical underpinning of rigor criteria and the tools shown here, including references cited, please follow this link.

  3. Version published to 10.1101/2021.09.27.462006v1 on bioRxiv