Integrated immunovirological profiling validates plasma SARS-CoV-2 RNA as an early predictor of COVID-19 mortality
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Abstract
Statistical models of plasma immunovirological features validate SARS-CoV-2 vRNA as an early predictor of COVID-19 mortality.
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SciScore for 10.1101/2021.03.18.21253907: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement Consent: The study was approved by the respective IRBs and written, informed consent obtained from all participants or, when incapacitated, their legal guardian before enrollment and sample collection. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable Median age and range for UC cohort was 37 (32–46), and 30 individuals were males (60%). Cell Line Authentication not detected. Table 2: Resources
Antibodies Sentences Resources Alexa Fluor-647-conjugated goat anti-human IgG (H+L) Abs (Invitrogen) were used as secondary antibodies to detect plasma binding in flow cytometry experiments. anti-human IgGsuggested: None… SciScore for 10.1101/2021.03.18.21253907: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement Consent: The study was approved by the respective IRBs and written, informed consent obtained from all participants or, when incapacitated, their legal guardian before enrollment and sample collection. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable Median age and range for UC cohort was 37 (32–46), and 30 individuals were males (60%). Cell Line Authentication not detected. Table 2: Resources
Antibodies Sentences Resources Alexa Fluor-647-conjugated goat anti-human IgG (H+L) Abs (Invitrogen) were used as secondary antibodies to detect plasma binding in flow cytometry experiments. anti-human IgGsuggested: NoneExperimental Models: Cell Lines Sentences Resources Flow cytometry analysis of cell-surface staining: Using the standard calcium phosphate method, 10 μg of SARS-CoV-2 Spike expressor and 2 μg of a green fluorescent protein (GFP) expressor (pIRES-GFP) were transfected into 2 × 106 293T cells. 293Tsuggested: NCBI_Iran Cat# C498, RRID:CVCL_0063)Software and Algorithms Sentences Resources All samples were acquired on an LSRII cytometer (BD Biosciences) and data analysis was performed using FlowJo v10.0.7 (Tree Star). FlowJosuggested: (FlowJo, RRID:SCR_008520)Results from OddPub: Thank you for sharing your code.
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
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