An engineered decoy receptor for SARS-CoV-2 broadly binds protein S sequence variants
This article has been Reviewed by the following groups
Listed in
- Evaluated articles (ScreenIT)
Abstract
An engineered decoy receptor tightly binds S from diverse SARS-related coronaviruses with limited potential for viral escape.
Article activity feed
-
-
-
SciScore for 10.1101/2020.10.18.344622: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Table 2: Resources
Antibodies Sentences Resources To measure competitive binding of wild type and engineered receptors, cells were instead incubated with 25 nM sACE22(WT)-IgG1 and 20 nM sACE22.v2.4-8h for 30 minutes at 4°C, washed twice, and stained with anti-human IgG-APC (clone HP6017, 1/250 dilution; BioLegend) and anti-HIS-FITC (chicken polyclonal, 1/100 dilution; Immunology Consultants Laboratory) secondary antibodies for 20 minutes at 4°C. anti-human IgG-APCsuggested: Noneanti-HIS-FITCsuggested: (Miltenyi Biotec Cat# 130-098-808, RRID:A…SciScore for 10.1101/2020.10.18.344622: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Table 2: Resources
Antibodies Sentences Resources To measure competitive binding of wild type and engineered receptors, cells were instead incubated with 25 nM sACE22(WT)-IgG1 and 20 nM sACE22.v2.4-8h for 30 minutes at 4°C, washed twice, and stained with anti-human IgG-APC (clone HP6017, 1/250 dilution; BioLegend) and anti-HIS-FITC (chicken polyclonal, 1/100 dilution; Immunology Consultants Laboratory) secondary antibodies for 20 minutes at 4°C. anti-human IgG-APCsuggested: Noneanti-HIS-FITCsuggested: (Miltenyi Biotec Cat# 130-098-808, RRID:AB_2751026)Software and Algorithms Sentences Resources Illumina sequencing data are deposited in NCBI’s Gene Expression Omnibus (GEO) under series accession number GSE159372. Gene Expression Omnibussuggested: (Gene Expression Omnibus (GEO, RRID:SCR_005012)Results from OddPub: Thank you for sharing your data.
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: We found the following clinical trial numbers in your paper:
Identifier Status Title NCT04427501 Recruiting A Study of LY3819253 (LY-CoV555) and LY3832479 (LY-CoV016) i… NCT04427501 Recruiting A Study of LY3819253 (LY-CoV555) and LY3832479 (LY-CoV016) i… Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: Please consider improving the rainbow (“jet”) colormap(s) used on pages 11 and 13. At least one figure is not accessible to readers with colorblindness and/or is not true to the data, i.e. not perceptually uniform.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
-