Hallmarks of Alpha- and Betacoronavirus non-structural protein 7+8 complexes
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Abstract
Coronaviral nsp7+8 complexes follow species- and stoichiometry-specific assembly pathways determined by few amino acid changes.
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SciScore for 10.1101/2020.09.30.320762: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis Cell pellets were lysed in 1:5 (v/v) buffer B1 (40 mM phosphate buffer, 300 mM NaCl, pH 8.0) with one freeze-thaw cycle, sonicated (micro tip, 70 % power, 6 times on 10 s, off 60 s; Branson digital sonifier SFX 150) and then centrifuged (20,000 × g for 45 min). Sex as a biological variable not detected. Table 2: Resources
Software and Algorithms Sentences Resources Raw data were calibrated with CsI (25 mg/mL) and analyzed using MassLynx 4.1 (Waters) MassLynxsuggested: (MassLynx , RRID:SCR_014271)Results from OddPub: Thank you for sharing your data.
Results from LimitationRecognizer: An explicit …SciScore for 10.1101/2020.09.30.320762: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement not detected. Randomization not detected. Blinding not detected. Power Analysis Cell pellets were lysed in 1:5 (v/v) buffer B1 (40 mM phosphate buffer, 300 mM NaCl, pH 8.0) with one freeze-thaw cycle, sonicated (micro tip, 70 % power, 6 times on 10 s, off 60 s; Branson digital sonifier SFX 150) and then centrifuged (20,000 × g for 45 min). Sex as a biological variable not detected. Table 2: Resources
Software and Algorithms Sentences Resources Raw data were calibrated with CsI (25 mg/mL) and analyzed using MassLynx 4.1 (Waters) MassLynxsuggested: (MassLynx , RRID:SCR_014271)Results from OddPub: Thank you for sharing your data.
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
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