A lymph node–targeted Amphiphile vaccine induces potent cellular and humoral immunity to SARS-CoV-2
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Abstract
Lymph node–targeting adjuvant AMP-CpG enhances SARS-CoV-2 RBD vaccine-induced cellular and humoral immune responses.
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SciScore for 10.1101/2020.08.17.251728: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement IACUC: All animal studies were carried out under an institute-approved IACUC protocol following federal, state, and local guidelines for the care and use of animals. Randomization Animals were not randomized for any of the studies, and dosing was not blinded. Blinding Animals were not randomized for any of the studies, and dosing was not blinded. Power Analysis not detected. Sex as a biological variable Animals: Female, 6 to 8-week-old C57BL/6J and BALB/c mice and 37-week-old C57BL/6J mice were purchased from Jackson Laboratory (Bar Harbor, ME). Cell Line Authentication not detected. Table 2: Resources
Antibodies Sentences Resources Antigen-Binding ELISA: ELISAs were performed … SciScore for 10.1101/2020.08.17.251728: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement IACUC: All animal studies were carried out under an institute-approved IACUC protocol following federal, state, and local guidelines for the care and use of animals. Randomization Animals were not randomized for any of the studies, and dosing was not blinded. Blinding Animals were not randomized for any of the studies, and dosing was not blinded. Power Analysis not detected. Sex as a biological variable Animals: Female, 6 to 8-week-old C57BL/6J and BALB/c mice and 37-week-old C57BL/6J mice were purchased from Jackson Laboratory (Bar Harbor, ME). Cell Line Authentication not detected. Table 2: Resources
Antibodies Sentences Resources Antigen-Binding ELISA: ELISAs were performed to determine sera antibody binding titers. Antigen-Binding ELISAsuggested: (Ling-Chu Hung / Animal Health Research Institute, Council of Agriculture, Executive Yuan, Taiwan Cat# 12-Hung-03 m& 20 ORF3 7D3, RRID:AB_2827541)Plates were washed 4 times with washing buffer (BioLegend; Cat: 4211601) and then incubated for 1 h at RT with a 1:2000 dilution of secondary antibody of horse radish peroxidase (HRP)-conjugated rabbit anti-mouse IgM (μ chain), anti-mouse IgM ( μ chain) ,suggested: NoneBriefly, bead populations with distinct fluorescence intensities that are coated with capture antibodies specific for various cytokines including IFNγ, TNFα, IL-4, IL-6, IL-10, and IL-17 were incubated with culture supernatants. TNFαsuggested: NoneIL-4suggested: NoneIL-6suggested: NoneIL-10suggested: NoneCells were stained with the following antibodies: PE anti-mouse IFNγ (BD, Cat: anti-mouse IFNγsuggested: NoneExperimental Models: Cell Lines Sentences Resources Control wells included ACE2-HEK293 cells or control HEK293 cells with the virus, but no sera, and provided the maximum transduction level and the background, respectively. HEK293suggested: CLS Cat# 300192/p777_HEK293, RRID:CVCL_0045)Pseudovirus neutralization data for the pilot experiment was performed by GenScript (Nanjing, China) following the same protocol, but using in-house ACE2-HEK293 cells and Spike RBD-HRP recombinant protein. ACE2-HEK293suggested: NoneExperimental Models: Organisms/Strains Sentences Resources Animals: Female, 6 to 8-week-old C57BL/6J and BALB/c mice and 37-week-old C57BL/6J mice were purchased from Jackson Laboratory (Bar Harbor, ME). C57BL/6Jsuggested: NoneBALB/csuggested: NoneSoftware and Algorithms Sentences Resources Mean fluorescent intensities (MFI) were calculated using FACSDiva software (BD) and protein concentrations were extrapolated using Microsoft Excel. FACSDivasuggested: (BD FACSDiva Software, RRID:SCR_001456)Microsoft Excelsuggested: (Microsoft Excel, RRID:SCR_016137)Sample acquisition was performed on FACSCanto II (BD) and data analyzed with FlowJo V10 software (TreeStar). FlowJosuggested: (FlowJo, RRID:SCR_008520)Cell pellets were re-suspended in 3 mL of ACK lysis buffer (Quality Biological, Inc., Cat: 118156101) for 5 min on ice; then PBS was added to stop the reaction. Quality Biologicalsuggested: NoneStatistics: All data were plotted and all statistical analyses were performed using GraphPad Prism 8 software (La Jolla, CA). GraphPad Prismsuggested: (GraphPad Prism, RRID:SCR_002798)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: Please consider improving the rainbow (“jet”) colormap(s) used on pages 27 and 28. At least one figure is not accessible to readers with colorblindness and/or is not true to the data, i.e. not perceptually uniform.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- No funding statement was detected.
- No protocol registration statement was detected.
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