ABO blood group does not influence the level of anti‐SARS‐CoV ‐2 antibodies in convalescent plasma donors
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Abstract
Background
The association of the ABO blood group with COVID‐19 disease has been confirmed by several studies, with the blood group A patients being more susceptible and prone to a more severe clinical course of the disease. Additionally, several authors also addressed the association of ABO‐types and the levels of anti‐SARS‐CoV‐2 antibodies in convalescents, mostly supporting a theory that the non‐O blood group convalescents present with higher levels of anti‐SARS‐CoV‐2 antibodies.
Study Design and Methods
Since previous findings were based on small convalescent cohorts, we quantified the anti‐SARS‐CoV‐2 antibody levels in a total of 3187 convalescent plasma donors with three commercial serological and one standard neutralizing antibody test. The majority of donors had undergone a mild form of the disease and the median time of sampling was 66 days after diagnosis.
Results
None of the antibody quantitation results showed any significant association with the ABO blood group types. The same result was evident in the subgroup of vaccinated individuals (n = 370) and the subgroups when stratified according to post‐COVID‐19 periods (0–60, 60–120, and 120–180 days).
Conclusion
In conclusion, we found no evidence to confirm that the ABO blood group types influence the level of SARS‐CoV‐2 antibody response in COVID‐19 convalescent plasma donors.
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SciScore for 10.1101/2021.10.19.21265195: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Ethics not detected. Sex as a biological variable not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Table 2: Resources
Antibodies Sentences Resources Serology testing: Two semi-quantitative and one quantitative serological tests were used to detect anti-SARS-CoV-2 antibodies: i) Wantai SARS-CoV-2 Ab ELISA, an enzyme-linked immunosorbent assay for qualitative detection of total IgG and IgM antibodies to the RBD of SARS-CoV-2 spike protein that was performed on 434 samples; ii) Abbott SARS-CoV-2 IgG assay, a chemiluminescent microparticle assay (CMIA) for the qualitative detection of IgG Abs to the nucleocapsid protein that was performed on 1019 samples; and iii) … SciScore for 10.1101/2021.10.19.21265195: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Ethics not detected. Sex as a biological variable not detected. Randomization not detected. Blinding not detected. Power Analysis not detected. Table 2: Resources
Antibodies Sentences Resources Serology testing: Two semi-quantitative and one quantitative serological tests were used to detect anti-SARS-CoV-2 antibodies: i) Wantai SARS-CoV-2 Ab ELISA, an enzyme-linked immunosorbent assay for qualitative detection of total IgG and IgM antibodies to the RBD of SARS-CoV-2 spike protein that was performed on 434 samples; ii) Abbott SARS-CoV-2 IgG assay, a chemiluminescent microparticle assay (CMIA) for the qualitative detection of IgG Abs to the nucleocapsid protein that was performed on 1019 samples; and iii) Abbott SARS-CoV-2 IgG II Quant, second generation CMIA for quantitative determination of IgG Abs to the RBD of the S1 subunit of the SARS-CoV-2 spike protein, including the neutralizing Abs that was performed on 2560 samples. anti-SARS-CoV-2suggested: NoneIgMsuggested: NoneResults from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- Thank you for including a funding statement. Authors are encouraged to include this statement when submitting to a journal.
- No protocol registration statement was detected.
Results from scite Reference Check: We found no unreliable references.
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