SARS‐CoV‐2 neutralization and serology testing of COVID‐19 convalescent plasma from donors with nonsevere disease
This article has been Reviewed by the following groups
Listed in
- Evaluated articles (ScreenIT)
Abstract
Background
The transfer of passive immunity with convalescent plasma is a promising strategy for treatment and prevention of COVID‐19, but donors with a history of nonsevere disease are serologically heterogenous. The relationship between SARS‐Cov‐2 antigen–binding activity and neutralization activity in this population of donors has not been defined.
Study Design and Methods
Convalescent plasma units from 47 individuals with a history of nonsevere COVID‐19 were assessed for antigen‐binding activity of using three clinical diagnostic serology assays (Beckman, DiaSorin, and Roche) with different SARS‐CoV‐2 targets. These results were compared with functional neutralization activity using a fluorescent reporter strain of SARS‐CoV‐2 in a microwell assay.
Results
Positive correlations of varying strength (Spearman r = 0.37‐0.52) between antigen binding and viral neutralization were identified. Donors age 48 to 75 years had the highest neutralization activity. Units in the highest tertile of binding activity for each assay were enriched (75%‐82%) for those with the highest levels of neutralization.
Conclusion
The strength of the relationship between antigen‐binding activity and neutralization varies depending on the clinical assay used. Units in the highest tertile of binding activity for each assay are predominantly comprised of those with the greatest neutralization activity.
Article activity feed
-
-
SciScore for 10.1101/2020.08.07.242271: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement IRB: This program and associated human subject research, performed in in accordance with the ethical standards of the Helsinki Declaration, were approved by the NorthShore University HealthSystem Institutional Review Board.
Consent: All potential donors provided written consent for the study and provided information about their COVID-19 disease history and demographics.Randomization Samples were divided into tertiles based on the Roche assay results, then 47 were randomly selected to equally sample each tertile. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Antibodies Se… SciScore for 10.1101/2020.08.07.242271: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement IRB: This program and associated human subject research, performed in in accordance with the ethical standards of the Helsinki Declaration, were approved by the NorthShore University HealthSystem Institutional Review Board.
Consent: All potential donors provided written consent for the study and provided information about their COVID-19 disease history and demographics.Randomization Samples were divided into tertiles based on the Roche assay results, then 47 were randomly selected to equally sample each tertile. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Antibodies Sentences Resources SARS-CoV-2 S1 RBD Ig ELISA: The anti-SARS-CoV-2 S1 RBD total Ig assay employed a standard indirect enzyme immunoassay technique (ELISA), described in detail elsewhere, using a secondary antibody recognizing all human immunoglobulin isotypes (goat anti-human IgG H+L-HRP, Invitrogen/ThermoFisher) [12,13] anti-SARS-CoV-2suggested: Noneanti-human IgG H+L-HRPsuggested: NoneExperimental Models: Cell Lines Sentences Resources Live SARS-CoV-2 Virus Neutralization Assay: Vero E6 cells (2.5×104) were seeded in each well of a 96-well Black/Clear Flat Bottom TC-treated plate (Falcon) and incubated in DMEM overnight at 37°C with 5% CO2 prior to infection. Vero E6suggested: NoneSoftware and Algorithms Sentences Resources A nonlinear regression method was used to determine the dilution that neutralized 50% of mNeonGreen fluorescence (NT50) by using Prism 8 (GraphPad). GraphPadsuggested: (GraphPad Prism, RRID:SCR_002798)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.Results from TrialIdentifier: No clinical trial numbers were referenced.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
Results from rtransparent:- Thank you for including a conflict of interest statement. Authors are encouraged to include this statement when submitting to a journal.
- No funding statement was detected.
- No protocol registration statement was detected.
-
SciScore for 10.1101/2020.08.07.242271: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement This program and associated human subject research, performed in in accordance with the ethical standards of the Helsinki Declaration, were approved by the NorthShore University HealthSystem Institutional Review Board. Randomization Samples were divided into tertiles based on the Roche assay results, then 47 were randomly selected to equally sample each tertile. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Antibodies Sentences Resources SARS-CoV-2 S1 RBD Ig ELISA The anti-SARS-CoV-2 S1 RBD total Ig assay employed a standard indirect enzyme immunoassay technique (ELISA), … SciScore for 10.1101/2020.08.07.242271: (What is this?)
Please note, not all rigor criteria are appropriate for all manuscripts.
Table 1: Rigor
Institutional Review Board Statement This program and associated human subject research, performed in in accordance with the ethical standards of the Helsinki Declaration, were approved by the NorthShore University HealthSystem Institutional Review Board. Randomization Samples were divided into tertiles based on the Roche assay results, then 47 were randomly selected to equally sample each tertile. Blinding not detected. Power Analysis not detected. Sex as a biological variable not detected. Cell Line Authentication not detected. Table 2: Resources
Antibodies Sentences Resources SARS-CoV-2 S1 RBD Ig ELISA The anti-SARS-CoV-2 S1 RBD total Ig assay employed a standard indirect enzyme immunoassay technique (ELISA), described in detail elsewhere, using a secondary antibody recognizing all human immunoglobulin isotypes (goat anti-human IgG H+L-HRP, Invitrogen/ThermoFisher) [12,13] anti-SARS-CoV-2suggested: Noneanti-human IgG H+L-HRPsuggested: NoneExperimental Models: Cell Lines Sentences Resources Live SARS-CoV-2 Virus Neutralization Assay Vero E6 cells (2.5×104) were seeded in each well of a 96-well Black/Clear Flat Bottom TC-treated plate (Falcon) and incubated in DMEM overnight at 37°C with 5% CO2 prior to infection. Vero E6suggested: NoneSoftware and Algorithms Sentences Resources A nonlinear regression method was used to determine the dilution that neutralized 50% of mNeonGreen fluorescence (NT50) by using Prism 8 (GraphPad). GraphPadsuggested: (GraphPad Prism, RRID:SCR_002798)Results from OddPub: We did not detect open data. We also did not detect open code. Researchers are encouraged to share open data when possible (see Nature blog).
Results from LimitationRecognizer: An explicit section about the limitations of the techniques employed in this study was not found. We encourage authors to address study limitations.
Results from Barzooka: We did not find any issues relating to the usage of bar graphs.
Results from JetFighter: We did not find any issues relating to colormaps.
About SciScore
SciScore is an automated tool that is designed to assist expert reviewers by finding and presenting formulaic information scattered throughout a paper in a standard, easy to digest format. SciScore checks for the presence and correctness of RRIDs (research resource identifiers), and for rigor criteria such as sex and investigator blinding. For details on the theoretical underpinning of rigor criteria and the tools shown here, including references cited, please follow this link.
-
