A ceramide synthase is important for filamentous fungal biofilm morphology and antifungal drug resistance

The complex structure of fungal biofilms generates microenvironments that impact the fitness of cells within the biofilm community. Contributions to fitness include the development of emergent properties resulting in the tolerance or resistance to external stressors such as rapid environmental changes and in the context of an infection, antifungal drug exposure. The biofilm developed by the filamentous fungal pathogen Aspergillus fumigatus develops zones of low oxygen which contribute to a reduction in antifungal drug susceptibility, however the genes and mechanisms involved in driving this emergent property of the biofilm are ill-defined. In this study, we utilized a transcriptomic approach to probe the biofilm structure in comparison to drug susceptible planktonic cultures to identify transcriptional patterns and genes unique to the A. fumigatus biofilm. Importantly we utilized two phenotypically diverse strains that allowed us to identify biofilm specific gene co-expression networks. One of these networks was highlighted by a gene encoding a ceramide synthase, designated barA , with a striking increase in barA transcript abundance specifically in the biofilm. Null mutants for barA in two strain backgrounds display altered biofilm morphology with some strain specific differences. Importantly, barA has a role in regulating susceptibility to ergosterol targeting antifungal drugs. These data identify biofilm specific genes in A. fumigatus for further study and highlight the importance of fungal ceramide synthases in mediating antifungal drug susceptibility in infection relevant biofilms.