Diazepam alters the shape of alpha oscillations recorded from human cortex using EEG

While neural oscillations are conventionally assessed via their frequency, power and phase, developing literature suggests that their shape also provides neurophysiological and functional information. However, the extent to which the shape of oscillations recorded non-invasively in humans index specific brain processes remains unclear. This study implemented a pharmaco-EEG approach to begin addressing this limitation. In 21 healthy adults, resting-state EEG data was collected before and after placebo or diazepam, a positive allosteric modulator of type A γ-aminobutyric acid (GABA _A ) receptors. The shape of individual cycles in the alpha band was then derived using empirical mode decomposition, followed by extraction of principal components (PCs) describing specific facets of alpha shape. Results of this approach show that all shape features were unchanged following placebo. In contrast, diazepam was associated with complex changes in several shape features, including peak-trough shape and edge speed. While changes in shape were apparent in all cortical lobes, the strongest alterations were specific to sensorimotor and parietal cortices. Taken together, our results support the neurophysiological utility of waveform shape, particularly with respect to non-invasive human recordings. Furthermore, the regional specificity of effects highlights the need for more granular exploration of waveform diversity.