Body-worn inertial measurement units track knee flexion angles with similar accuracy to optical motion capture

Knee motion is altered in overuse injuries and chronic diseases like osteoarthritis. Yet, relying on marker-based motion capture in the lab limits our understanding of how knee motion continuously impacts joint health in the real world. Markerless optical motion capture improves clinical suitability, but space and time constraints remain barriers toward real-world assessments. Inertial measurement unit (IMU) sensors have enabled continuous motion tracking outside the lab. In this study, we recorded thigh and shank-worn IMU data concurrently with marker-based and markerless optical motion capture on 10 healthy adults, who performed various daily living and exercise movements. We developed an IMU virtual alignment and data fusion paradigm to estimate knee flexion angle during each movement. We compared IMU-based estimate against marker-based and markerless motion capture using Pearson correlation (R _xy ) and root-mean-square difference (RMSD). IMU-estimated knee flexion angle strongly correlated with motion capture (R _xy ≥ 0.9). RMSDs were small for slower movements like walking, stairs, and squats (RMSD = 4.4° – 6.0°) while larger during faster movements like running and jumping (RMSD = 5.4° – 9.4°). Our findings show that wearable IMUs track knee flexion with similar accuracy to optical motion capture during daily living activities typical to older adults, highlighting their potential for monitoring real-world mobility in knees with chronic diseases. Conversely, it remains inconclusive whether IMUs accurately track dynamic knee motion relevant to athletic injuries. Future research should seek best practice for IMU wearing and mitigate practical pitfalls to secure high-fidelity data, for identifying clinically meaningful real-world biomarkers of knee mobility.