Specificity of the stabilising interaction between intrinsically disordered protein sequences and G-quadruplexes in RNA

Intrinsically disordered regions (IDRs) are present in and essential for the function of nearly all the proteins involved in the regulation, cell and developmental processes. The RGG domain in IDRs binds ′promiscuously′ to RNA G-quadruplexes (rG4s), a non-canonical 4-stranded secondary structure that occurs in many transcripts involved in gene regulation. Here we show, using weak binding interactions between a minimal RGG-rich peptide and rG4s, that the IDR selectively templates and stabilises the structure of the human telomeric TERRA rG4, providing a unique pathway to RNA folding that does not rely on high-affinity binding or monovalent cations. Multidimensional NMR and CD analyses reveal individual nucleotide and amino acid identities determine the specificity of the interaction between RGG peptides and rG4s, explaining how IDRs can selectively recognise RNA over DNA G4s, the high specificity of such interactions in vivo and the high frequency of monogenic mutations observed in IDRs.