Genome-wide characterization of clonal hematopoiesis reveals extensive non-coding putative driver mutations

As humans age, we acquire somatic mutations in our blood, leading to clonal hematopoiesis (CH). Despite the prevalence of clonal hematopoiesis (CH) in aged individuals, recent searches for selective sweeps in single-cell derived colonies have revealed that most clones have expanded without a known driver mutation. This extensive, unexplained CH motivated our search for novel driver mutations across ∼490K blood whole genome sequences from the UK Biobank. We searched across variants with a minor allele count of at least 10 (∼147M variants) to discover alleles that are enriched in aged individuals. We identified 45 variants including known CH driver genes (e.g., DNMT3A , ASXL1, SF3B1 ) and 35 novel variants. Among the novel age-associated variants, we identified 72 carriers of a somatic mutation in the TERT promoter. Although previously reported in pan-cancer analyses, TERT promoter mutations have typically been excluded from population searches for CH. We also observed a somatic intronic insertion in UGT2B7 in 1,165 carriers, a cluster of IGH point mutations, and centromeric variation. We conducted a phenome-wide association study (PheWAS) among 30 common disease phenotypes to characterize the phenotypic correlates of these mutations, finding that non-canonical CH mutations contributed ∼47% of liability scale heritability. We then performed a genome-wide association study of the IGH mutations, finding that common germline variation at GRAMD1B is strongly associated with IGH mutations. Overall, we characterize CH at both increased breadth and resolution and characterize the entire cascade from upstream germline risk haplotypes to downstream clinical correlates. We release our summary statistics in a publicly accessible portal, somatic.emory.edu.