Small- and large-scale patches shape benthic microbial community structure and function in streams at the subcontinental scale

Streams and rivers process dissolved and particulate matter as water moves along the land-to-ocean continuum, making important contributions to global biogeochemical cycles. Yet, predicting stream and river microbial metabolism associated with biogeochemical transformations at broad spatial scales remains challenging. Here, we used data from the National Ecological Observatory Network program to investigate whether ecological relationships among microbial community structure and function and environmental conditions observed at small scales hold at the subcontinental scale. We found that microbial communities were best explained by site-specific conditions. However, when field replicates were averaged, stream physico-chemical characteristics such as pH and temperature emerged as driving factors. This indicates that water quality acted as an environmental filter on microbial communities at the subcontinental scale, but was masked by small-scale patches that created high spatial heterogeneity. Our findings underscore the importance of considering multiple spatial scales to fully understand benthic microbial communities’ role in stream biogeochemistry.