Proteome integral solubility alteration assay for monitoring antibody-antigen cell interactions
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The Proteome Integral Solubility Alteration (PISA) assay is commonly used to identify and characterize interactions between small molecules and the cellular proteome. In this study, a modified PISA approach identified tumor necrosis factor alpha (TNFα) as the antigen of the monoclonal antibody infliximab in activated monocytes - uniquely among ∼8,000 cellular proteins. These results highlight the potential of PISA-based analysis not only for monitoring antibody-antigen interactions in therapeutic development, but also for identifying new antigens and characterizing the initial effector functions of antibodies within complex proteome environments.
Teaser
Modified PISA pinpointed TNF-α as Infliximab’s antigen among 8000 proteins, highlighting broad potential for therapeutic antigen studies.
